Our understanding of the genetic mechanisms that underlie biological processes has relied
extensively on loss-of-function (LOF) analyses. LOF methods target DNA, RNA or protein to …

The nematode Caenorhabditis elegans is now a major model organism in biology. The
choice of Sydney Brenner to adopt this species in the mid-1960s and the success of his …

DNA transposons have been widely used for transgenesis and insertional mutagenesis in
various organisms. Among the transposons active in mammalian cells, the moth-derived …

Double-stranded (ds) RNA can induce sequence-specific inhibition of gene function in
several organisms. However, both the mechanism and the physiological role of the …

Background RNA interference (RNAi) is a highly conserved cellular mechanism. In some
organisms, such as Caenorhabditis elegans, the RNAi response can be transmitted …

We devised an efficient genetic mapping system in the nematode Caenorhabditis elegans
which is based upon the differences in number and location of the transposable element Tc1 …

Genome-wide analysis of gene function is essential for the post-genome era, and
development of efficient and economical technology suitable for it has been in demand …

In Caenorhabditis elegans, the introduction of double-stranded RNA triggers sequence-
specific genetic interference (RNAi) that is transmitted to offspring. The inheritance …

We have used an antisense strategy to effectively disrupt the expression of two genes
encoding myofilament proteins present in C. elegans body wall muscles. DNA segments …

The mec. 3 gene is essential for proper differentiation of the set of six touch receptor neurons
in C. elegans. In mutants lacking mec. 3 activity, the touch receptors express none of their …