Visualizing and discovering cellular structures with super-resolution microscopy

YM Sigal, R Zhou, X Zhuang - Science, 2018 - science.org
Super-resolution microscopy has overcome a long-held resolution barrier—the diffraction
limit—in light microscopy and enabled visualization of previously invisible molecular details …

STED super-resolved microscopy

G Vicidomini, P Bianchini, A Diaspro - Nature methods, 2018 - nature.com
Stimulated emission depletion (STED) microscopy provides subdiffraction resolution while
preserving useful aspects of fluorescence microscopy, such as optical sectioning, and …

Fluorescence nanoscopy in cell biology

SJ Sahl, SW Hell, S Jakobs - Nature reviews Molecular cell biology, 2017 - nature.com
Fluorescence nanoscopy uniquely combines minimally invasive optical access to the
internal nanoscale structure and dynamics of cells and tissues with molecular detection …

Expansion microscopy: principles and uses in biological research

AT Wassie, Y Zhao, ES Boyden - Nature methods, 2019 - nature.com
Many biological investigations require 3D imaging of cells or tissues with nanoscale spatial
resolution. We recently discovered that preserved biological specimens can be physically …

Stimulated emission depletion microscopy

H Blom, J Widengren - Chemical reviews, 2017 - ACS Publications
Despite its short history, diffraction-unlimited fluorescence microscopy techniques have
already made a substantial imprint in the biological sciences. In this review, we describe …

Quantum metrology and its application in biology

MA Taylor, WP Bowen - Physics Reports, 2016 - Elsevier
Quantum metrology provides a route to overcome practical limits in sensing devices. It holds
particular relevance to biology, where sensitivity and resolution constraints restrict …

Technological advances in super-resolution microscopy to study cellular processes

C Bond, AN Santiago-Ruiz, Q Tang, M Lakadamyali - Molecular Cell, 2022 - cell.com
Since its initial demonstration in 2000, far-field super-resolution light microscopy has
undergone tremendous technological developments. In parallel, these developments have …

[HTML][HTML] Breaking the diffraction barrier: super-resolution imaging of cells

B Huang, H Babcock, X Zhuang - Cell, 2010 - cell.com
Anyone who has used a light microscope has wished that its resolution could be a little
better. Now, after centuries of gradual improvements, fluorescence microscopy has made a …

A guide to super-resolution fluorescence microscopy

L Schermelleh, R Heintzmann, H Leonhardt - Journal of Cell Biology, 2010 - rupress.org
For centuries, cell biology has been based on light microscopy and at the same time been
limited by its optical resolution. However, several new technologies have been developed …

An introduction to optical super-resolution microscopy for the adventurous biologist

J Vangindertael, R Camacho, W Sempels… - … and applications in …, 2018 - iopscience.iop.org
Ever since the inception of light microscopy, the laws of physics have seemingly thwarted
every attempt to visualize the processes of life at its most fundamental, sub-cellular, level …