The MCM motor of the replicative helicase is loaded onto origin DNA as an inactive double
hexamer before replication initiation. Recruitment of activators GINS and Cdc45 upon S …

The paradigm of cellular systems as deterministic machines has long guided our
understanding of biology. Advancements in technology and methodology, however, have …

In eukaryotes, DNA replication initiation requires assembly and activation of the
minichromosome maintenance (MCM) 2–7 double hexamer (DH) to melt origin DNA …

The coupling of chromatography with mass spectrometry (MS) has becoming a routine
technique in proteomics for protein primary sequence and post translational modification …

The replisome that replicates the eukaryotic genome consists of at least three engines: the
Cdc45-MCM-GINS (CMG) helicase that separates duplex DNA at the replication fork and …

There is currently a lack of efficacious treatments for patients with chemo-resistant small-cell
lung cancer (SCLC), leading to poor prognoses. We examined a chemo-resistant SCLC cell …

Activation of the replicative Mcm2-7 helicase by loading GINS and Cdc45 is crucial for
replication origin firing, and as such for faithful genetic inheritance. Our biochemical and …

Simple Summary To ensure the maintenance of genetic stability prior to cell division a cell's
complement of chromosomes must be duplicated. This requires not only the replication of …

The cryo-electron microscopy (cryoEM) method has enabled high-resolution structure
determination of numerous biomolecules and complexes. Nevertheless, cryoEM sample …

DNA replication initiation in eukaryotes is tightly regulated through two cell-cycle specific
processes, replication licensing to install inactive minichromosome maintenance (MCM) …