A review of progress in single particle tracking: from methods to biophysical insights

C Manzo, MF Garcia-Parajo - Reports on progress in physics, 2015 - iopscience.iop.org
Optical microscopy has for centuries been a key tool to study living cells with minimum
invasiveness. The advent of single molecule techniques over the past two decades has …

Unraveling the thousand word picture: an introduction to super-resolution data analysis

A Lee, K Tsekouras, C Calderon, C Bustamante… - Chemical …, 2017 - ACS Publications
Super-resolution microscopy provides direct insight into fundamental biological processes
occurring at length scales smaller than light's diffraction limit. The analysis of data at such …

Mean square displacement analysis of single-particle trajectories with localization error: Brownian motion in an isotropic medium

X Michalet - Physical Review E—Statistical, Nonlinear, and Soft …, 2010 - APS
We examine the capability of mean square displacement (MSD) analysis to extract reliable
values of the diffusion coefficient D of a single particle undergoing Brownian motion in an …

Actin restricts FcɛRI diffusion and facilitates antigen-induced receptor immobilization

NL Andrews, KA Lidke, JR Pfeiffer, AR Burns… - Nature cell …, 2008 - nature.com
The actin cytoskeleton has been implicated in restricting diffusion of plasma membrane
components. Here, simultaneous observations of quantum dot-labelled FcɛRI motion and …

Three-dimensional tracking of single mRNA particles in Saccharomyces cerevisiae using a double-helix point spread function

MA Thompson, JM Casolari… - Proceedings of the …, 2010 - National Acad Sciences
Optical imaging of single biomolecules and complexes in living cells provides a useful
window into cellular processes. However, the three-dimensional dynamics of most important …

Tracking single molecules in the live cell plasma membrane—Do's and Don't's

S Wieser, GJ Schütz - Methods, 2008 - Elsevier
In recent years, the development of fast and highly sensitive microscopy has changed the
way of thinking of cell biologists: it became more and more important to study the structural …

Stochastic particle unbinding modulates growth dynamics and size of transcription factor condensates in living cells

G Muñoz-Gil, C Romero-Aristizabal… - Proceedings of the …, 2022 - National Acad Sciences
Liquid–liquid phase separation (LLPS) is emerging as a key physical principle for biological
organization inside living cells, forming condensates that play important regulatory roles …

Dependence of DNA persistence length on ionic strength of solutions with monovalent and divalent salts: a joint theory–experiment study

A Brunet, C Tardin, L Salomé, P Rousseau… - Biophysical …, 2016 - cell.com
Chemistry, University of Illinois at Chicago, Chicago, IL, USA. The evaluation of nucleotide
photoionization threshold energies provides useful information for understanding …

Separating actin-dependent chemokine receptor nanoclustering from dimerization indicates a role for clustering in CXCR4 signaling and function

L Martínez-Muñoz, JM Rodríguez-Frade, R Barroso… - Molecular cell, 2018 - cell.com
A current challenge in cell motility studies is to understand the molecular and physical
mechanisms that govern chemokine receptor nanoscale organization at the cell membrane …

CRISPR-dCas13-tracing reveals transcriptional memory and limited mRNA export in developing zebrafish embryos

Y Huang, BQ Gao, Q Meng, LZ Yang, XK Ma, H Wu… - Genome Biology, 2023 - Springer
Background Understanding gene transcription and mRNA-protein (mRNP) dynamics in
single cells in a multicellular organism has been challenging. The catalytically dead …