Portable nanopore-sequencing technology: Trends in development and applications

P Chen, Z Sun, J Wang, X Liu, Y Bai, J Chen… - Frontiers in …, 2023 - frontiersin.org
Sequencing technology is the most commonly used technology in molecular biology
research and an essential pillar for the development and applications of molecular biology …

Plassembler: an automated bacterial plasmid assembly tool

G Bouras, AE Sheppard, V Mallawaarachchi… - …, 2023 - academic.oup.com
With recent advances in sequencing technologies, it is now possible to obtain near-perfect
complete bacterial chromosome assemblies cheaply and efficiently by combining a long …

Scalable and versatile container-based pipelines for de novo genome assembly and bacterial annotation

FM de Almeida, TA de Campos… - F1000Research, 2023 - pmc.ncbi.nlm.nih.gov
Background: Advancements in DNA sequencing technology have transformed the field of
bacterial genomics, allowing for faster and more cost effective chromosome level …

Porechop_ABI: discovering unknown adapters in Oxford Nanopore Technology sequencing reads for downstream trimming

Q Bonenfant, L Noé, H Touzet - Bioinformatics Advances, 2023 - academic.oup.com
Abstract Motivation Oxford Nanopore Technologies (ONT) sequencing has become very
popular over the past few years and offers a cost-effective solution for many genomic and …

Hybracter: enabling scalable, automated, complete and accurate bacterial genome assemblies

G Bouras, G Houtak, RR Wick… - Microbial …, 2024 - microbiologyresearch.org
Improvements in the accuracy and availability of long-read sequencing mean that complete
bacterial genomes are now routinely reconstructed using hybrid (ie short-and long-reads) …

Comparison of Illumina and Oxford Nanopore Technology for genome analysis of Francisella tularensis, Bacillus anthracis, and Brucella suis

J Linde, H Brangsch, M Hölzer, C Thomas… - BMC genomics, 2023 - Springer
Background Bacterial epidemiology needs to understand the spread and dissemination of
strains in a One Health context. This is important for highly pathogenic bacteria such as …

Nanopore sequencing for de novo bacterial genome assembly and search for single-nucleotide polymorphism

MG Khrenova, TV Panova, VA Rodin… - International Journal of …, 2022 - mdpi.com
Nanopore sequencing (ONT) is a new and rapidly developing method for determining
nucleotide sequences in DNA and RNA. It serves the ability to obtain long reads of …

Dynamic changes in Shiga toxin (Stx) 1 transducing phage throughout the evolution of O26:H11 Stx-producing Escherichia coli

B Yano, I Taniguchi, Y Gotoh, T Hayashi… - Scientific reports, 2023 - nature.com
Shiga toxin (Stx) is the key virulence factor of Stx-producing Escherichia coli (STEC). All
known Stxs (Stx1 and Stx2) are encoded by bacteriophages (Stx phages). Although the …

Molecular characterization of an outbreak-involved Bacillus anthracis strain confirms the spillover of anthrax from West Africa

S Wang, R Suluku, MB Jalloh, AF Samba… - Infectious Diseases of …, 2024 - Springer
Background Anthrax, a zoonotic disease caused by the spore-forming bacterium Bacillus
anthracis, remains a major global public health concern, especially in countries with limited …

Alteration of a Shiga toxin-encoding phage associated with a change in toxin production level and disease severity in Escherichia coli

T Miyata, I Taniguchi, K Nakamura… - Microbial …, 2023 - microbiologyresearch.org
Among the nine clades of Shiga toxin (Stx)-producing Escherichia coli O157: H7, clade 8 is
thought to be highly pathogenic, as it causes severe disease more often than other clades …