Ultrahigh-Throughput Enzyme Engineering and Discovery in In Vitro Compartments

M Gantz, S Neun, EJ Medcalf, LD van Vliet… - Chemical …, 2023 - ACS Publications
Novel and improved biocatalysts are increasingly sourced from libraries via experimental
screening. The success of such campaigns is crucially dependent on the number of …

A practical guide to preparation and applications of giant unilamellar vesicles formed via centrifugation of water-in-oil emulsion droplets

Y Zhang, H Obuchi, T Toyota - Membranes, 2023 - mdpi.com
Giant vesicles (GVs), which are closed lipid bilayer membranes with a diameter of more than
1 μm, have attracted attention not only as model cell membranes but also for the …

Computational DNA droplets recognizing miRNA sequence inputs based on liquid–liquid phase separation

J Gong, N Tsumura, Y Sato… - Advanced Functional …, 2022 - Wiley Online Library
Phase‐separated biomolecular droplets are formed in cells to regulate various biological
processes. This phenomenon can be applied to constructing self‐assembled dynamic …

Capsule-like DNA hydrogels with patterns formed by lateral phase separation of DNA nanostructures

Y Sato, M Takinoue - JACS Au, 2021 - ACS Publications
Phase separation is a key phenomenon in artificial cell construction. Recent studies have
shown that the liquid–liquid phase separation of designed-DNA nanostructures induces the …

[HTML][HTML] Preparation and biomedical applications of artificial cells

Q Xu, Z Zhang, PPY Lui, L Lu, X Li, X Zhang - Materials Today Bio, 2023 - Elsevier
Artificial cells have received much attention in recent years as cell mimics with typical
biological functions that can be adapted for therapeutic and diagnostic applications, as well …

A large, square-shaped, DNA origami nanopore with sealing function on a giant vesicle membrane

S Iwabuchi, I Kawamata, S Murata… - Chemical …, 2021 - pubs.rsc.org
Intaking molecular information from the external environment is essential for the normal
functioning of artificial cells/molecular robots. Herein, we report the design and function of a …

Rapid and facile preparation of giant vesicles by the droplet transfer method for artificial cell construction

Y Shimane, Y Kuruma - Frontiers in Bioengineering and …, 2022 - frontiersin.org
Giant vesicles have been widely used for the bottom-up construction of artificial (or synthetic)
cells and the physicochemical analysis of lipid membranes. Although methods for the …

Amplification of over 100 kbp DNA from single template molecules in femtoliter droplets

H Ueno, H Sawada, N Soga, M Sano, S Nara… - ACS Synthetic …, 2021 - ACS Publications
Reconstitution of the DNA amplification system in microcompartments is the primary step
toward artificial cell construction through a bottom-up approach. However, amplification of> …

Recent advances in liposome-based molecular robots

K Shoji, R Kawano - Micromachines, 2020 - mdpi.com
A molecular robot is a microorganism-imitating micro robot that is designed from the
molecular level and constructed by bottom-up approaches. As with conventional robots …

A Self‐Regenerating Artificial Cell, that is One Step Closer to Living Cells: Challenges and Perspectives

S Tror, SM Jeon, HT Nguyen, E Huh, K Shin - Small Methods, 2023 - Wiley Online Library
Controllable, self‐regenerating artificial cells (SRACs) can be a vital advancement in the
field of synthetic biology, which seeks to create living cells by recombining various biological …