An improved imaging system that corrects MS2-induced RNA destabilization

W Li, A Maekiniemi, H Sato, C Osman, RH Singer - Nature methods, 2022 - nature.com
The MS2 and MS2-coat protein (MS2-MCP) imaging system is widely used to study
messenger RNA (mRNA) spatial distribution in living cells. Here, we report that the MS2 …

The acyltransferase Gpc1 is both a target and an effector of the unfolded protein response in Saccharomyces cerevisiae

VL Hrach, WR King, LD Nelson, S Conklin… - Journal of Biological …, 2023 - ASBMB
The unfolded protein response (UPR) is sensitive to proteotoxic and membrane bilayer
stress, both of which are sensed by the ER protein Ire1. When activated, Ire1 splices HAC1 …

分子伴侣增强蛋白酶K 在毕赤酵母中的表达及对羊毛鳞片层的作用分析.

蔡逸安, 张轶群, 杨子璇, 刘业学, 刘文龙… - Biotechnology …, 2024 - search.ebscohost.com
[目的] 通过分子伴侣共表达增强毕赤酵母中异源蛋白酶K 的分泌水平, 并对其的羊毛无氯剥鳞
作用机制进行解析, 旨在提高蛋白酶K 的表达量并为高效酶法剥鳞技术的应用奠定基础.[方法] …

[图书][B] Shedding Light on mRNA Localization in S. cerevisiae: Mechanisms and Methodologies

A Maekiniemi - 2023 - search.proquest.com
Subcellular mRNA localization has emerged as an essential determinant of gene
expression regulation in space and time. The budding yeast, S. cerevisiae, offers an …