Caveat fluorophore: an insiders' guide to small-molecule fluorescent labels

JB Grimm, LD Lavis - Nature Methods, 2022 - nature.com
The last three decades have brought a revolution in fluorescence microscopy. The
development of new microscopes, fluorescent labels and analysis techniques has pushed …

Chemical approaches to optimize the properties of organic fluorophores for imaging and sensing

G Jiang, H Liu, H Liu, G Ke, TB Ren… - Angewandte …, 2024 - Wiley Online Library
Organic fluorophores are indispensable tools in cells, tissue and in vivo imaging, and have
enabled much progress in the wide range of biological and biomedical fields. However …

Targeted multicolor in vivo imaging over 1,000 nm enabled by nonamethine cyanines

VG Bandi, MP Luciano, M Saccomano, NL Patel… - Nature …, 2022 - nature.com
Recent progress has shown that using wavelengths between 1,000 and 2,000 nm, referred
to as the shortwave-infrared or near-infrared (NIR)-II range, can enable high-resolution in …

Method to diversify cyanine chromophore functionality enables improved biomolecule tracking and intracellular imaging

SM Usama, SC Marker, DH Li… - Journal of the …, 2023 - ACS Publications
Heptamethine indocyanines are invaluable probes for near-infrared (NIR) imaging. Despite
broad use, there are only a few synthetic methods to assemble these molecules, and each …

Bright Chromenylium Polymethine Dyes Enable Fast, Four-Color In Vivo Imaging with Shortwave Infrared Detection

ED Cosco, BA Arús, AL Spearman… - Journal of the …, 2021 - ACS Publications
Optical imaging within the shortwave infrared (SWIR, 1000–2000 nm) region of the
electromagnetic spectrum has enabled high-resolution and high-contrast imaging in mice …

Doubly Strapped Zwitterionic NIR‐I and NIR‐II Heptamethine Cyanine Dyes for Bioconjugation and Fluorescence Imaging

DH Li, RS Gamage, AG Oliver, NL Patel… - Angewandte …, 2023 - Wiley Online Library
Heptamethine cyanine dyes enable deep tissue fluorescence imaging in the near infrared
(NIR) window. Small molecule conjugates of the benchmark dye ZW800‐1 have been tested …

AND-gate contrast agents for enhanced fluorescence-guided surgery

JC Widen, M Tholen, JJ Yim, A Antaris… - Nature biomedical …, 2021 - nature.com
Surgical resection of tumours requires precisely locating and defining the margins between
lesions and normal tissue. However, this is made difficult by irregular margin borders …

Sterically shielded heptamethine cyanine dyes for bioconjugation and high performance near‐infrared fluorescence imaging

DH Li, CL Schreiber, BD Smith - Angewandte Chemie, 2020 - Wiley Online Library
The near‐infrared window of fluorescent heptamethine cyanine dyes greatly facilitates
biological imaging because there is deep penetration of the light and negligible background …

Near-infrared heptamethine cyanines (Cy7): from structure, property to application

L Feng, W Chen, X Ma, SH Liu, J Yin - Organic & Biomolecular …, 2020 - pubs.rsc.org
Heptamethine cyanine dyes (Cy7) have attracted much attention in the field of biological
application due to their unique structure and attractive near infrared (NIR) photophysical …

Deciphering the structure–property relations in substituted heptamethine cyanines

L Stackova, E Muchova, M Russo… - The Journal of …, 2020 - ACS Publications
Heptamethine cyanines (Cy7) are fluorophores essential for modern bioimaging techniques
and chemistry. Here, we systematically evaluated the photochemical and photophysical …