Evaluating phase separation in live cells: diagnosis, caveats, and functional consequences

DT McSwiggen, M Mir, X Darzacq… - Genes & …, 2019 - genesdev.cshlp.org
The idea that liquid–liquid phase separation (LLPS) may be a general mechanism by which
molecules in the complex cellular milieu may self-organize has generated much excitement …

Spatial proteomics: a powerful discovery tool for cell biology

E Lundberg, GHH Borner - Nature Reviews Molecular Cell Biology, 2019 - nature.com
Protein subcellular localization is tightly controlled and intimately linked to protein function in
health and disease. Capturing the spatial proteome—that is, the localizations of proteins and …

OpenCell: Endogenous tagging for the cartography of human cellular organization

NH Cho, KC Cheveralls, AD Brunner, K Kim… - Science, 2022 - science.org
Elucidating the wiring diagram of the human cell is a central goal of the postgenomic era.
We combined genome engineering, confocal live-cell imaging, mass spectrometry, and data …

[HTML][HTML] Integrated intracellular organization and its variations in human iPS cells

MP Viana, J Chen, TA Knijnenburg, R Vasan, C Yan… - Nature, 2023 - nature.com
Understanding how a subset of expressed genes dictates cellular phenotype is a
considerable challenge owing to the large numbers of molecules involved, their …

Uncovering the mode of action of engineered T cells in patient cancer organoids

JF Dekkers, M Alieva, A Cleven, F Keramati… - Nature …, 2023 - nature.com
Extending the success of cellular immunotherapies against blood cancers to the realm of
solid tumors will require improved in vitro models that reveal therapeutic modes of action at …

Membrane and organelle dynamics during cell division

JG Carlton, H Jones, US Eggert - Nature Reviews Molecular Cell …, 2020 - nature.com
During division, eukaryotic cells undergo a dramatic, complex and coordinated remodelling
of their cytoskeleton and membranes. For cell division to occur, chromosomes must be …

Genetically encoded multimeric tags for subcellular protein localization in cryo-EM

HKH Fung, Y Hayashi, VT Salo, A Babenko, I Zagoriy… - Nature …, 2023 - nature.com
Cryo-electron tomography (cryo-ET) allows for label-free high-resolution imaging of
macromolecular assemblies in their native cellular context. However, the localization of …

Chromatin structure dynamics during the mitosis-to-G1 phase transition

H Zhang, DJ Emerson, TG Gilgenast, KR Titus, Y Lan… - Nature, 2019 - nature.com
Features of higher-order chromatin organization—such as A/B compartments, topologically
associating domains and chromatin loops—are temporarily disrupted during mitosis …

miRNATissueAtlas2: an update to the human miRNA tissue atlas

A Keller, L Gröger, T Tschernig, J Solomon… - Nucleic acids …, 2022 - academic.oup.com
Small non-coding RNAs (sncRNAs) are pervasive regulators of physiological and
pathological processes. We previously developed the human miRNA Tissue Atlas, detailing …

Self-supervised deep learning encodes high-resolution features of protein subcellular localization

H Kobayashi, KC Cheveralls, MD Leonetti, LA Royer - Nature methods, 2022 - nature.com
Explaining the diversity and complexity of protein localization is essential to fully understand
cellular architecture. Here we present cytoself, a deep-learning approach for fully self …