Evaluating phase separation in live cells: diagnosis, caveats, and functional consequences
The idea that liquid–liquid phase separation (LLPS) may be a general mechanism by which
molecules in the complex cellular milieu may self-organize has generated much excitement …
molecules in the complex cellular milieu may self-organize has generated much excitement …
Spatial proteomics: a powerful discovery tool for cell biology
E Lundberg, GHH Borner - Nature Reviews Molecular Cell Biology, 2019 - nature.com
Protein subcellular localization is tightly controlled and intimately linked to protein function in
health and disease. Capturing the spatial proteome—that is, the localizations of proteins and …
health and disease. Capturing the spatial proteome—that is, the localizations of proteins and …
OpenCell: Endogenous tagging for the cartography of human cellular organization
Elucidating the wiring diagram of the human cell is a central goal of the postgenomic era.
We combined genome engineering, confocal live-cell imaging, mass spectrometry, and data …
We combined genome engineering, confocal live-cell imaging, mass spectrometry, and data …
[HTML][HTML] Integrated intracellular organization and its variations in human iPS cells
Understanding how a subset of expressed genes dictates cellular phenotype is a
considerable challenge owing to the large numbers of molecules involved, their …
considerable challenge owing to the large numbers of molecules involved, their …
Uncovering the mode of action of engineered T cells in patient cancer organoids
JF Dekkers, M Alieva, A Cleven, F Keramati… - Nature …, 2023 - nature.com
Extending the success of cellular immunotherapies against blood cancers to the realm of
solid tumors will require improved in vitro models that reveal therapeutic modes of action at …
solid tumors will require improved in vitro models that reveal therapeutic modes of action at …
Membrane and organelle dynamics during cell division
JG Carlton, H Jones, US Eggert - Nature Reviews Molecular Cell …, 2020 - nature.com
During division, eukaryotic cells undergo a dramatic, complex and coordinated remodelling
of their cytoskeleton and membranes. For cell division to occur, chromosomes must be …
of their cytoskeleton and membranes. For cell division to occur, chromosomes must be …
Genetically encoded multimeric tags for subcellular protein localization in cryo-EM
Cryo-electron tomography (cryo-ET) allows for label-free high-resolution imaging of
macromolecular assemblies in their native cellular context. However, the localization of …
macromolecular assemblies in their native cellular context. However, the localization of …
Chromatin structure dynamics during the mitosis-to-G1 phase transition
Features of higher-order chromatin organization—such as A/B compartments, topologically
associating domains and chromatin loops—are temporarily disrupted during mitosis …
associating domains and chromatin loops—are temporarily disrupted during mitosis …
miRNATissueAtlas2: an update to the human miRNA tissue atlas
A Keller, L Gröger, T Tschernig, J Solomon… - Nucleic acids …, 2022 - academic.oup.com
Small non-coding RNAs (sncRNAs) are pervasive regulators of physiological and
pathological processes. We previously developed the human miRNA Tissue Atlas, detailing …
pathological processes. We previously developed the human miRNA Tissue Atlas, detailing …
Self-supervised deep learning encodes high-resolution features of protein subcellular localization
Explaining the diversity and complexity of protein localization is essential to fully understand
cellular architecture. Here we present cytoself, a deep-learning approach for fully self …
cellular architecture. Here we present cytoself, a deep-learning approach for fully self …