The madness of microbiome: attempting to find consensus “best practice” for 16S microbiome studies

J Pollock, L Glendinning, T Wisedchanwet… - Applied and …, 2018 - Am Soc Microbiol
The development and continuous improvement of high-throughput sequencing platforms
have stimulated interest in the study of complex microbial communities. Currently, the most …

Ecosystems monitoring powered by environmental genomics: a review of current strategies with an implementation roadmap

T Cordier, L Alonso‐Sáez… - Molecular …, 2021 - Wiley Online Library
A decade after environmental scientists integrated high‐throughput sequencing
technologies in their toolbox, the genomics‐based monitoring of anthropogenic impacts on …

RESCRIPt: Reproducible sequence taxonomy reference database management

MS Robeson, DR O'Rourke, BD Kaehler… - PLoS computational …, 2021 - journals.plos.org
Nucleotide sequence and taxonomy reference databases are critical resources for
widespread applications including marker-gene and metagenome sequencing for …

EPA-ng: massively parallel evolutionary placement of genetic sequences

P Barbera, AM Kozlov, L Czech, B Morel… - Systematic …, 2019 - academic.oup.com
Next generation sequencing (NGS) technologies have led to a ubiquity of molecular
sequence data. This data avalanche is particularly challenging in metagenetics, which …

AutoMLST: an automated web server for generating multi-locus species trees highlighting natural product potential

M Alanjary, K Steinke, N Ziemert - Nucleic Acids Research, 2019 - academic.oup.com
Understanding the evolutionary background of a bacterial isolate has applications for a wide
range of research. However generating an accurate species phylogeny remains …

Navigating the seven challenges of taxonomic reference databases in metabarcoding analyses

F Keck, M Couton, F Altermatt - Molecular Ecology Resources, 2023 - Wiley Online Library
Assessment of biodiversity using metabarcoding data, such as from bulk or environmental
DNA sampling, is becoming increasingly relevant in ecology, biodiversity sciences and …

Genesis and Gappa: processing, analyzing and visualizing phylogenetic (placement) data

L Czech, P Barbera, A Stamatakis - Bioinformatics, 2020 - academic.oup.com
We present genesis, a library for working with phylogenetic data, and gappa, an
accompanying command-line tool for conducting typical analyses on such data. The tools …

[HTML][HTML] Anatomy promotes neutral coexistence of strains in the human skin microbiome

A Conwill, AC Kuan, R Damerla, AJ Poret, JS Baker… - Cell Host & Microbe, 2022 - cell.com
What enables strains of the same species to coexist in a microbiome? Here, we investigate
whether host anatomy can explain strain co-residence of Cutibacterium acnes, the most …

Prospects and challenges of implementing DNA metabarcoding for high-throughput insect surveillance

AM Piper, J Batovska, NOI Cogan, J Weiss… - …, 2019 - academic.oup.com
Trap-based surveillance strategies are widely used for monitoring of invasive insect species,
aiming to detect newly arrived exotic taxa as well as track the population levels of …

Parasites dominate hyperdiverse soil protist communities in Neotropical rainforests

F Mahé, C de Vargas, D Bass, L Czech… - Nature ecology & …, 2017 - nature.com
High animal and plant richness in tropical rainforest communities has long intrigued
naturalists. It is unknown if similar hyperdiversity patterns are reflected at the microbial scale …