Proteins are the main targets of most drugs; however, system-wide methods to monitor
protein activity and function are still underused in drug discovery. Novel biochemical …

Proteins adopt different higher-order structures (HOS) to enable their unique biological
functions. Understanding the complexities of protein higher-order structures and dynamics …

Mass spectrometry is a central technology in the life sciences, providing our most
comprehensive account of the molecular inventory of the cell. In parallel with developments …

Solution-phase hydrogen/deuterium exchange (HDX) coupled to mass spectrometry (MS) is
a widespread tool for structural analysis across academia and the biopharmaceutical …

Functional regulation of ligand-activated receptors is driven by alterations in the
conformational dynamics of the protein upon ligand binding. Differential hydrogen/deuterium …

▪ Abstract Mass spectrometry has provided a powerful method for monitoring hydrogen
exchange of protein backbone amides with deuterium from solvent. In comparison to …

Thermodynamic stability is fundamental to the biology of proteins. Information on protein
stability is essential for studying protein structure and folding and can also be used indirectly …

Following sequencing and assembly of the human genome, the preferred methods for
identification of new drug targets have changed dramatically. Modern tactics such as …

Ion mobility spectrometry, with subsequent mass spectrometric detection, has been
employed to study the stability of compact protein conformations of FK-binding protein, hen …

Most knowledge of protein structure and function is derived from experiments performed with
purified protein resuspended in dilute, buffered solutions. However, proteins function in the …