A guide to genome engineering with programmable nucleases

H Kim, JS Kim - Nature Reviews Genetics, 2014 - nature.com
Programmable nucleases—including zinc-finger nucleases (ZFNs), transcription activator-
like effector nucleases (TALENs) and RNA-guided engineered nucleases (RGENs) derived …

Plant genome engineering for targeted improvement of crop traits

KEM Sedeek, A Mahas, M Mahfouz - Frontiers in plant science, 2019 - frontiersin.org
To improve food security, plant biology research aims to improve crop yield and tolerance to
biotic and abiotic stress, as well as increasing the nutrient contents of food. Conventional …

RNA‐guided transcriptional regulation in planta via synthetic dCas9‐based transcription factors

A Piatek, Z Ali, H Baazim, L Li… - Plant biotechnology …, 2015 - Wiley Online Library
Targeted genomic regulation is a powerful approach to accelerate trait discovery and
development in agricultural biotechnology. Bacteria and archaea use clustered regularly …

Behind the lines–actions of bacterial type III effector proteins in plant cells

D Büttner - FEMS microbiology reviews, 2016 - academic.oup.com
Pathogenicity of most Gram-negative plant-pathogenic bacteria depends on the type III
secretion (T3S) system, which translocates bacterial effector proteins into plant cells. Type III …

Ralstonia solanacearum, a widespread bacterial plant pathogen in the post‐genomic era

N Peeters, A Guidot, F Vailleau… - Molecular plant …, 2013 - Wiley Online Library
R alstonia solanacearum is a soil‐borne bacterium causing the widespread disease known
as bacterial wilt. R alstonia solanacearum is also the causal agent of M oko disease of …

Oh, the places they'll go! A survey of phytopathogen effectors and their host targets

M Khan, D Seto, R Subramaniam… - The Plant …, 2018 - Wiley Online Library
Phytopathogens translocate effector proteins into plant cells where they sabotage the host
cellular machinery to promote infection. An individual pathogen can translocate numerous …

[HTML][HTML] Tailored biosynthesis of gibberellin plant hormones in yeast

KR Kildegaard, JA Arnesen, B Adiego-Pérez… - Metabolic …, 2021 - Elsevier
The application of small amounts of natural plant growth hormones, such as gibberellins
(GAs), can increase the productivity and quality of many vegetable and fruit crops. However …

Genome engineering via TALENs and CRISPR/Cas9 systems: challenges and perspectives

MM Mahfouz, A Piatek… - Plant biotechnology …, 2014 - Wiley Online Library
The ability to precisely modify genome sequence and regulate gene expression patterns in
a site‐specific manner holds much promise in plant biotechnology. Genome‐engineering …

TALEN-mediated genome editing: prospects and perspectives

DA Wright, T Li, B Yang, MH Spalding - Biochemical Journal, 2014 - portlandpress.com
Genome editing is the practice of making predetermined and precise changes to a genome
by controlling the location of DNA DSBs (double-strand breaks) and manipulating the cell's …

Genome editing: the road of CRISPR/Cas9 from bench to clinic

A Eid, MM Mahfouz - Experimental & Molecular Medicine, 2016 - nature.com
Molecular scissors engineered for site-specific modification of the genome hold great
promise for effective functional analyses of genes, genomes and epigenomes and could …