Blood and lymphatic vasculatures on-chip platforms and their applications for organ-specific in vitro modeling

AR Henderson, H Choi, E Lee - Micromachines, 2020 - mdpi.com
The human circulatory system is divided into two complementary and different systems, the
cardiovascular and the lymphatic system. The cardiovascular system is mainly concerned …

A bioengineered lymphatic vessel model for studying lymphatic endothelial cell‐cell junction and barrier function

AR Henderson, IS Ilan, E Lee - Microcirculation, 2021 - Wiley Online Library
Abstract Objective Lymphatic vessels (LVs) maintain fluid homeostasis by draining interstitial
fluid. A failure in lymphatic drainage triggers lymphatic diseases such as lymphedema …

Engineered models of the lymphatic vascular system: past, present, and future

A Selahi, A Jain - Microcirculation, 2023 - Wiley Online Library
The lymphatic vascular system is crucial for optimizing body fluid level, regulating immune
function, and transporting lipid. Relative to the experimental models to investigate blood …

A 3D human lymphatic vessel-on-chip reveals the roles of interstitial flow and VEGF-A/C for lymphatic sprouting and discontinuous junction formation

IS Ilan, AR Yslas, Y Peng, R Lu, E Lee - Cellular and Molecular …, 2023 - Springer
Abstract Introduction Lymphatic vessels (LVs) maintain fluid homeostasis by draining excess
interstitial fluid, which is accomplished by two distinct LVs: initial LVs and collecting LVs. The …

A human initial lymphatic chip reveals distinct mechanisms of primary lymphatic valve dysfunction in acute and chronic inflammation

S Kraus, E Lee - Lab on a Chip, 2023 - pubs.rsc.org
Interstitial fluid uptake and retention by lymphatic vessels (LVs) play a role in maintaining
interstitial fluid homeostasis. While it is well-established that intraluminal lymphatic valves in …

Isolation and short‐term culturing of primary lymphatic endothelial cells from collecting lymphatics: A techniques study

KL Jablon, VL Akerstrom, M Li, SE Braun… - …, 2023 - Wiley Online Library
Objective To develop an experimental method for routine isolation and short‐term culture of
primary lymphatic endothelial cells from specific collecting vessels. Methods Lymphatic …

Lymphatic endothelial cell-targeting lipid nanoparticles delivering VEGFC mRNA improve lymphatic function after injury

E Michalaki, R Chin, K Jeong, Z Qi, LN Liebman… - …, 2024 - pmc.ncbi.nlm.nih.gov
Dysfunction of the lymphatic system following injury, disease, or cancer treatment can lead
to lymphedema, a debilitating condition with no cure. Advances in targeted therapy have …

Lymphatic tissue and organ engineering for in vitro modeling and in vivo regeneration

AM Kolarzyk, G Wong, E Lee - Cold Spring …, 2022 - perspectivesinmedicine.cshlp.org
The lymphatic system has an important role in maintaining fluid homeostasis and
transporting immune cells and biomolecules, such as dietary fat, metabolic products, and …

Engineering blood and lymphatic microvascular networks

A Crnic, S Rohringer, T Tyschuk, W Holnthoner - Atherosclerosis, 2024 - Elsevier
The human vasculature plays a crucial role in the blood supply of nearly all organs as well
as the drainage of the interstitial fluid. Consequently, if these physiological systems go awry …

Advances in skin-on-a-chip and skin tissue engineering

A Pappalardo, A Cespedes, HL Vu, HE Abaci - Principles of Human Organs …, 2023 - Elsevier
Abstract Development of physiologically relevant in vitro skin models that allow for collecting
reliable and efficient data can help us better understand the human skin and address the …