Marine antifreeze proteins: structure, function, and application to cryopreservation as a potential cryoprotectant

HJ Kim, JH Lee, YB Hur, CW Lee, SH Park, BW Koo - Marine drugs, 2017 - mdpi.com
Antifreeze proteins (AFPs) are biological antifreezes with unique properties, including
thermal hysteresis (TH), ice recrystallization inhibition (IRI), and interaction with membranes …

Potential importance of vitrification in reproductive medicine

J Liebermann, F Nawroth, V Isachenko… - Biology of …, 2002 - academic.oup.com
As early as 1985, ice-free cryopreservation of mouse embryos at− 196° C by vitrification was
reported in an attempted alternative approach to cryostorage. Since then, vitrification …

Sugars exert a major influence on the vitrification properties of ethylene glycol-based solutions and have low toxicity to embryos and oocytes

LL Kuleshova, DR Macfarlane, AO Trounson, JM Shaw - Cryobiology, 1999 - Elsevier
A systematic approach was taken to assess the vitrification properties of ethylene glycol-
based solutions supplemented with carbohydrates. Solutions were prepared by weight …

Terminology associated with vitrification and other cryopreservation procedures for oocytes and embryos

JM Shaw, GM Jones - Human reproduction update, 2003 - academic.oup.com
Over the past 30 years many cryopreservation procedures have been applied to oocytes,
embryos, sperm, ovarian and testicular tissue. Over this time many, often specialized, terms …

Thermal hysteresis proteins

J Barrett - The international journal of biochemistry & cell biology, 2001 - Elsevier
Extreme environments present a wealth of biochemical adaptations. Thermal hysteresis
proteins (THPs) have been found in vertebrates, invertebrates, plants, bacteria and fungi …

The use of antifreeze proteins in the cryopreservation of gametes and embryos

V Robles, D G. Valcarce, M F. Riesco - Biomolecules, 2019 - mdpi.com
The cryopreservation of gametes and embryos is a technique widely used in reproductive
biology. This technology helps in the reproductive management of domesticated animals …

Cryopreservation of bovinein VitroProduced embryos using ethylene glycol in controlled freezing or vitrification

V Sommerfeld, H Niemann - Cryobiology, 1999 - Elsevier
In this study, the cryoprotectant ethylene glycol (EG) was tested for its ability to improve and
facilitate the cryopreservation ofin vitroproduced (IVP) bovine embryos. Embryos were …

Blastocyst development after vitrification of multipronuclear zygotes using the Flexipet denuding pipette

J Liebermann, MJ Tucker, JR Graham, T Han… - Reproductive …, 2002 - Elsevier
The purpose of this study was to demonstrate the safety and efficacy of vitrification of human
pronuclear stage (PN) embryos in the human assisted reproduction laboratory. Using single …

Comparison of ethylene glycol, 1, 2-propanediol and glycerol for cryopreservation of slow-cooled mouse zygotes, 4-cell embryos and blastocysts

S Emiliani, M Van den Bergh, AS Vannin… - Human …, 2000 - academic.oup.com
The aim of the study was to analyse the toxicity, the osmolar and cryoprotective activity of
ethylene glycol (ETG) in terms of survival rate (SR), cleavage rate (CR) and expanded …

Cryopreservation of human embryonic stem cells without the use of a programmable freezer

SY Ha, BC Jee, CS Suh, HS Kim, SK Oh… - Human …, 2005 - academic.oup.com
BACKGROUND: An effective freezing–thawing technique is crucial for the clinical
application of human embryonic stem (ES) cells. The aim of this study was to find an optimal …