kallisto, bustools and kb-python for quantifying bulk, single-cell and single-nucleus RNA-seq

DK Sullivan, KH Min, KE Hjörleifsson, L Luebbert… - Nature …, 2024 - nature.com
The term 'RNA-seq'refers to a collection of assays based on sequencing experiments that
involve quantifying RNA species from bulk tissue, single cells or single nuclei. The kallisto …

A fast, reproducible, high-throughput variant calling workflow for population genomics

CD Mirchandani, AJ Shultz… - Molecular Biology …, 2024 - academic.oup.com
The increasing availability of genomic resequencing data sets and high-quality reference
genomes across the tree of life present exciting opportunities for comparative population …

Efficient querying of genomic reference databases with gget

L Luebbert, L Pachter - Bioinformatics, 2023 - academic.oup.com
Motivation A recurring challenge in interpreting genomic data is the assessment of results in
the context of existing reference databases. With the increasing number of command line …

Tracing the invertebrate herpesviruses in the global sequence datasets

U Rosani, M Gaia, TO Delmont… - Frontiers in Marine …, 2023 - frontiersin.org
The family of Malacoherpesviridae is currently represented by only two viruses infecting
molluscs, Ostreid herpesvirus 1 (OsHV-1) and Haliotid herpesvirus 1 (HaHV-1), both …

GEOfetch: a command-line tool for downloading data and standardized metadata from GEO and SRA

O Khoroshevskyi, N LeRoy, VP Reuter… - …, 2023 - academic.oup.com
Abstract Motivation The Gene Expression Omnibus has become an important source of
biological data for secondary analysis. However, there is no simple, programmatic way to …

A bipartite function of ESRRB can integrate signaling over time to balance self-renewal and differentiation

TE Knudsen, WB Hamilton, M Proks, M Lykkegaard… - Cell Systems, 2023 - cell.com
Cooperative DNA binding of transcription factors (TFs) integrates the cellular context to
support cell specification during development. Naive mouse embryonic stem cells are …

Quantifying orthogonal barcodes for sequence census assays

AS Booeshaghi, KH Min, J Gehring… - Bioinformatics …, 2024 - academic.oup.com
Barcode-based sequence census assays utilize custom or random oligonucloetide
sequences to label various biological features, such as cell-surface proteins or CRISPR …

Genome plasticity shapes the ecology and evolution of Phocaeicola dorei and Phocaeicola vulgatus

E Da Silva Morais, GM Grimaud, A Warda, C Stanton… - Scientific Reports, 2024 - nature.com
Phocaeicola dorei and Phocaeicola vulgatus are very common and abundant members of
the human gut microbiome and play an important role in the infant gut microbiome. These …

KARAJ: An Efficient Adaptive Multi-Processor Tool to Streamline Genomic and Transcriptomic Sequence Data Acquisition

M Labani, A Beheshti, NH Lovell… - International Journal of …, 2022 - mdpi.com
Here we developed KARAJ, a fast and flexible Linux command-line tool to automate the end-
to-end process of querying and downloading a wide range of genomic and transcriptomic …

Challenges to sharing sample metadata in computational genomics

NC Sheffield, NJ LeRoy, O Khoroshevskyi - Frontiers in Genetics, 2023 - frontiersin.org
The genomic data deluge has led to challenges with sharing and integrating genomic data.
While substantial effort has been devoted to making genomics data easier to share, one …