Analysing soil microbial communities is often hampered by DNA adsorption on soil organic
compounds such as humic acids. However the role of humic acids in DNA adsorption and …

We quantified both DNA and humic acid concentrations during the extraction and
purification of DNA from compost. The DNA extraction method consisted of bead-beating …

Sensitive PCR detection of viral nucleic acids plays a critical role in infectious disease
research, diagnosis and monitoring. In the context of SARS-CoV-2 detection, recent reports …

The concentration of free DNA in soils has to be determined in order to understand the fate
and the transport of extracellular DNA. A protocol for the extraction and determination of free …

The effectiveness of three commercially available direct DNA isolation kits (Mobio, Fast,
Qiagen) and one published direct DNA extraction protocol (Bead) for extracting bacterial …

Candidatus Microtrhix parvicella is one of the most common filamentous bacteria reported to
be involved in bulking and foaming problems in activated sludge plants worldwide. In order …

We examined the soil properties affecting adsorption of plasmid DNA and its transformation
efficiency using competent Escherichia coli cells in sterile forested Andosols (n= 2), arable …

The contamination of agricultural land and groundwater by heavy metals is essentially
linked to human activities. A major problem with heavy metals is that they cannot be …

A commercial electric paint shaker was utilized to enable the simultaneous extraction of
DNA from multiple large soil samples. A bead homogenization procedure was used to …

The nitrate-nitrogen concentration in soil is estimated based on the nitrate-nitrogen 200 nm
absorption peak. In one embodiment, a device measures the attenuation spectrum of a soil …