High-resolution DNA melting analysis for simple and efficient molecular diagnostics
GH Reed, JO Kent, CT Wittwer - Pharmacogenomics, 2007 - Taylor & Francis
High-resolution melting of DNA is a simple solution for genotyping, mutation scanning and
sequence matching. The melting profile of a PCR product depends on its GC content, length …
sequence matching. The melting profile of a PCR product depends on its GC content, length …
Real-time multiplex PCR assays
CT Wittwer, MG Herrmann, CN Gundry… - Methods, 2001 - Elsevier
The ability to multiplex PCR by probe color and melting temperature (Tm) greatly expands
the power of real-time analysis. Simple hybridization probes with only a single fluorescent …
the power of real-time analysis. Simple hybridization probes with only a single fluorescent …
High-resolution genotyping by amplicon melting analysis using LCGreen
CT Wittwer, GH Reed, CN Gundry… - Clinical …, 2003 - academic.oup.com
Background: High-resolution amplicon melting analysis was recently introduced as a closed-
tube method for genotyping and mutation scanning (Gundry et al. Clin Chem 2003; 49: 396 …
tube method for genotyping and mutation scanning (Gundry et al. Clin Chem 2003; 49: 396 …
Replacing PCR with COLD-PCR enriches variant DNA sequences and redefines the sensitivity of genetic testing
PCR is widely employed as the initial DNA amplification step for genetic testing. However, a
key limitation of PCR-based methods is the inability to selectively amplify low levels of …
key limitation of PCR-based methods is the inability to selectively amplify low levels of …
Amplicon melting analysis with labeled primers: a closed-tube method for differentiating homozygotes and heterozygotes
CN Gundry, JG Vandersteen, GH Reed… - Clinical …, 2003 - academic.oup.com
Background: Common methods for identification of DNA sequence variants use gel
electrophoresis or column separation after PCR. Methods: We developed a method for …
electrophoresis or column separation after PCR. Methods: We developed a method for …
Characterization of EvaGreen and the implication of its physicochemical properties for qPCR applications
F Mao, WY Leung, X Xin - BMC biotechnology, 2007 - Springer
Background EvaGreen (EG) is a newly developed DNA-binding dye that has recently been
used in quantitative real-time PCR (qPCR), post-PCR DNA melt curve analysis and several …
used in quantitative real-time PCR (qPCR), post-PCR DNA melt curve analysis and several …
Amplicon DNA melting analysis for mutation scanning and genotyping: cross-platform comparison of instruments and dyes
MG Herrmann, JD Durtschi, LK Bromley… - Clinical …, 2006 - academic.oup.com
Background: DNA melting analysis for genotyping and mutation scanning of PCR products
by use of high-resolution instruments with special “saturation” dyes has recently been …
by use of high-resolution instruments with special “saturation” dyes has recently been …
Oxytocin receptor genetic variation promotes human trust behavior
Given that human trust behavior is heritable and intranasal administration of oxytocin
enhances trust, the oxytocin receptor (OXTR) gene is an excellent candidate to investigate …
enhances trust, the oxytocin receptor (OXTR) gene is an excellent candidate to investigate …
[HTML][HTML] Strategies for improving detection of circulating tumor DNA using next generation sequencing
R Tébar-Martínez, J Martín-Arana… - Cancer Treatment …, 2023 - Elsevier
Cancer has become a global health issue and liquid biopsy has emerged as a non-invasive
tool for various applications. In cancer, circulating tumor DNA (ctDNA) can be detected from …
tool for various applications. In cancer, circulating tumor DNA (ctDNA) can be detected from …
High resolution melting analysis of almond SNPs derived from ESTs
High resolution melting curve (HRM) is a recent advance for the detection of SNPs. The
technique measures temperature induced strand separation of short PCR amplicons, and is …
technique measures temperature induced strand separation of short PCR amplicons, and is …