Precise spatio–temporal control of gene activity is essential for organismal development,
growth, and survival in a changing environment. Decisive steps in gene regulation involve …

Abstract RNA polymerase II (RNA Pol II) speed or elongation rate, ie, the number of
nucleotides synthesized per unit of time, is a major determinant of transcriptome …

During gene transcription, RNA polymerase II (RNAPII) traverses nucleosomes in chromatin,
but the mechanism has remained elusive. Using cryo–electron microscopy, we obtained …

Gene regulation involves activation of RNA polymerase II (Pol II) that is paused and bound
by the protein complexes DRB sensitivity-inducing factor (DSIF) and negative elongation …

Recent advances in sequencing techniques that measure nascent transcripts and that
reveal the positioning of RNA polymerase II (Pol II) have shown that the pausing of Pol II in …

Understanding how splicing events are coordinated across numerous introns in metazoan
RNA transcripts requires quantitative analyses of transient RNA processing events in living …

The elongation stage of transcription by RNA polymerase II (RNA Pol II) is central to the
regulation of gene expression in response to developmental and environmental cues in …

Pre-mRNA maturation frequently occurs at the same time and place as transcription by RNA
polymerase II. The co-transcriptionality of mRNA processing has permitted the evolution of …

To dampen proteotoxic stresses and maintain protein homeostasis, organisms possess a
stress-responsive molecular machinery that detects and neutralizes protein damage. A …

Elongation is becoming increasingly recognized as a critical step in eukaryotic
transcriptional regulation. Although traditional genetic and biochemical studies have …