In vitro cell cultures are an important tool for obtaining insights into cellular processes in an
isolated system and a supplement to in vivo animal experiments. While primary dissociated …

Slices of CNS tissue prepared from young rodents can be maintained in culture for many
weeks to months. The basic requirements are simple: a stable substratum, culture medium …

Time-lapse fluorescence confocal microscopy was used to directly visualize the formation
and dynamics of postsynaptic target structures (ie, dendritic branches and spines) on …

This article describes current experimental models of status epilepticus (SE) and neuronal
injury for use in the screening of new therapeutic agents. Epilepsy is a common neurological …

Summary Arc/Arg3. 1 is an immediate-early gene whose expression levels are increased by
strong synaptic activation, including synapse-strengthening activity patterns. Arc/Arg3. 1 …

In vitro models of traumatic brain injury (TBI) are helping elucidate the pathobiological
mechanisms responsible for dysfunction and delayed cell death after mechanical stimulation …

We monitored developmental alterations in the morphology of dendritic spines in primary
cultures of hippocampal neurons using confocal laser scanning microscopy (CLSM) and the …

Recent improvements in brain slice technology have made this biological preparation
increasingly useful for examining pathophysiology of brain diseases in a tissue context …

Traumatic brain injury (TBI) initiates a cascade of numerous pathophysiological events that
evolve over time. Despite the complexity of TBI, research aimed at therapy development has …

Protoplasmic astrocytes in mammalian CNS tissues in vivo have a highly complex 3D
morphology, but in dissociated cell cultures they often assume a flattened, fibroblast-like …