With the growing popularity of serine/threonine ligation (STL) and cysteine/penicillamine
ligation (CPL) in chemical protein synthesis, facile and general approaches for the …

Enantioselective reduction of cyclopropenyl esters and ketones to optically active
cyclopropanes has been achieved by using whole-cell-overexpressing ene-reductases …

It is desired to design and construct more efficient enzymes with better performance to
catalyze carbene N–H insertions for the synthesis of bioactive molecules. To this end, we …

The incorporation of non-canonical amino acids (ncAAs) into the metal coordination
environments of proteins has endowed metalloproteins with enhanced properties and novel …

Chloramphenicols (CAPs) are ubiquitous emerging pollutants that threaten ecological
environments and human health. Microbial and enzyme-based biodegradation strategies …

In this study, we developed a machine-learning-aided protein design strategy for
engineering Vitreoscilla hemoglobin (VHb) as carbene transferase. A Natural Language …

Organoborons have recently received much attention, while a biocatalytic platform for the
synthesis of chiral organoborons is limited only to Rma cytochrome c. In this study, we …

Herein, we report a semi-synthetic strategy affording a nitrophorin 2 (NP2) variant with a N,
N′-bis (2-pyridylmethyl) amine (Dpa) ligand as sidechain selectively installed at position …

In this study, we aimed to develop protein‐based metal ligands to catalyze cis‐selective
cyclopropanation using the TM1459 cupin protein superfamily. Copper complexes with …

In the past decade, carbene transfer biocatalysis has evolved from basic scientific research
to an area with vast potential for the development of new industrial processes in the …