The presence of unrepaired lesions in DNA represents a challenge for replication. Most, but
not all, DNA lesions block the replicative DNA polymerases. The conceptually simplest …

DNA damage by chemicals, radiation, or oxidative stress leads to a mutational spectrum,
which is complex because it is determined in part by lesion structure, the DNA sequence …

Dealing with DNA lesions during genome replication is particularly challenging because
damaged replication templates interfere with the progression of the replicative DNA …

During replication, the presence of unrepaired lesions results in the formation of single
stranded DNA (ssDNA) gaps that need to be repaired to preserve genome integrity and cell …

Cells cope with replication-blocking lesions via translesion DNA synthesis (TLS). TLS is
carried out by low-fidelity DNA polymerases that replicate across lesions, thereby preventing …

Lesion tolerance pathways allow cells to proceed with replication despite the presence of
replication-blocking lesions in their genome. Following transient fork stalling, replication …

DNA lesions can block replication forks and lead to the formation of single-stranded gaps.
These replication complications are mitigated by DNA damage tolerance mechanisms …

Escherichia coli DNA polymerase IV (Pol IV, also known as DinB) is a Y-family DNA
polymerase capable of catalyzing translesion DNA synthesis (TLS) on certain DNA lesions …

DNA damage tolerance pathways allow cells to duplicate their genomes despite the
presence of replication blocking lesions. Cells possess two major tolerance strategies …

Processivity clamps tether DNA polymerases to DNA, allowing their access to the primer–
template junction. In addition to DNA replication, DNA polymerases also participate in …