In-cell structural biology aims at extracting structural information about proteins or nucleic
acids in their native, cellular environment. This emerging field holds great promise and is …

A detailed knowledge of the complex processes that make cells and organisms alive is
fundamental in order to understand diseases and to develop novel drugs and therapeutic …

NMR spectroscopy is currently extensively used in binding assays for hit identification, but its
use in dissociation constant determination is more limited when compared to other …

Abstract Background Traditional Chinese medicine (TCM), as a significant part of the global
pharmaceutical science, the abundant molecular compounds it contains is a valuable …

In-cell NMR spectroscopy is a powerful approach to study protein structure and function in
the native cellular environment. It provides precious insights into the folding, maturation …

Here, we report for the first time a series of sulfonamide derivatives with scaffolds bearing
flexible moieties, namely, rotamers or tropoisomers capable of adapting their geometry in …

The cellular environment can affect the structure and function of pharmacological targets
and the interaction with potential drugs. Such complexity is often overlooked in the first steps …

In-cell NMR, ie, NMR spectroscopy applied to studying specific macromolecules within living
cells, is becoming the technique of choice for the structural and mechanistic description of …

A library of novel alkyl/benzyl (4-sulphamoylphenyl) carbamimidothioates was synthesised
by selective S-alkylation of the easily accessible 4-thioureidobenzenesulphonamide. The …

The development of more effective drugs requires knowledge of their bioavailability and
binding efficacy directly in the native cellular environment. In-cell nuclear magnetic …