Abstract RNA polymerase II (RNA Pol II) speed or elongation rate, ie, the number of
nucleotides synthesized per unit of time, is a major determinant of transcriptome …

Blood and immune cells derive from multipotent hematopoietic stem cells (HSCs).
Classically, stem and progenitor populations have been considered discrete homogeneous …

Visualizing the location and dynamics of RNAs in live cells is key to understanding their
function. Here, we identify two endonuclease-deficient, single-component programmable …

A long-standing question in gene regulation is how remote enhancers communicate with
their target promoters, and specifically how chromatin topology dynamically relates to gene …

Transcription is episodic, consisting of a series of discontinuous bursts. Using live-imaging
methods and quantitative analysis, we examine transcriptional bursting in living Drosophila …

Reconstructing the lineage relationships and dynamic event histories of individual cells
within their native spatial context is a long-standing challenge in biology. Many biological …

RNA processing plays a central role in accurately transmitting genetic information into
functional RNA and protein regulators. To fully appreciate the RNA life-cycle, tools to …

Translation is the fundamental biological process converting mRNA information into
proteins. Single-molecule imaging in live cells has illuminated the dynamics of RNA …

The programmes that direct an organism's development and maintenance are encoded in its
genome. Decoding of this information begins with regulated transcription of genomic DNA …

Maturation of mRNA precursors often occurs simultaneously with their synthesis by RNA
polymerase II (Pol II). The co-transcriptional nature of mRNA processing has permitted the …