Droplet-based microfluidic systems generate, manipulate and control sub-microlitre droplets
enclosed within an immiscible carrier fluid. Owing to a number of remarkable features, such …

Biocatalysis has undergone revolutionary progress in the past century. Benefited by the
integration of multidisciplinary technologies, natural enzymatic reactions are constantly …

Novel and improved biocatalysts are increasingly sourced from libraries via experimental
screening. The success of such campaigns is crucially dependent on the number of …

Single-cell RNA sequencing has recently emerged as a powerful tool for mapping cellular
heterogeneity in diseased and healthy tissues, yet high-throughput methods are needed for …

Enzymes are versatile catalysts and their synthetic potential has been recognized for a long
time. In order to exploit their full potential, enzymes often need to be re-engineered or …

Droplet-based microfluidic systems have been employed to manipulate discrete fluid
volumes with immiscible phases. Creating the fluid droplets at microscale has led to a …

There is a recognized and growing need for rapid and efficient cell assays, where the size of
microfluidic devices lend themselves to the manipulation of cellular populations down to the …

We present a droplet-based microfluidics protocol for high-throughput analysis and sorting
of single cells. Compartmentalization of single cells in droplets enables the analysis of …

The ability to manipulate and sort droplets is a fundamental issue in droplet-based
microfluidics. Various lab-on-a-chip applications can only be realized if droplets are …

Designing catalysts that achieve the rates and selectivities of natural enzymes is a long-
standing goal in protein chemistry. Here, we show that an ultrahigh-throughput droplet …