Cystic fibrosis (CF) is an autosomal recessive disease caused by mutations in the cystic
fibrosis transmembrane conductance regulator (CFTR) gene that encodes a cAMP …

The chemical modification of adenovirus (Ad) gene transfer vectors with synthetic polymers
is a promising strategy for overcoming typical in vivo hurdles associated with Ad-mediated …

The human airway epithelium forms a barrier between the external and internal
environments, separating air from the interstitial space. However, it also serves many other …

Pulmonary epithelial cells are continuously exposed to microbial challenges as a result of
breathing. It is recognized that immune myeloid cells express Toll-like receptors (TLRs) …

Dysfunction of CFTR in cystic fibrosis (CF) airway epithelium perturbs the normal regulation
of ion transport, leading to a reduced volume of airway surface liquid (ASL), mucus …

Systemic delivery of adenoviral vectors is a major goal in cancer gene therapy, but is
currently prohibited by rapid hepatic uptake of virus following intravenous injection with …

Abstract Knowledge of genetic origins of obstructive lung diseases has made inhaled gene
therapy an attractive alternative to the current standards of care that are limited to managing …

Clinical trials of gene therapy for cystic fibrosis suggest that current levels of gene transfer
efficiency are probably too low to result in clinical benefit, largely as a result of the barriers …

Background Interest in airways inflammatory disease has increasingly focused on innate
immunity. We investigated several components of innate immunity in induced sputum of …

Replication-defective vectors based on human adenovirus serotypes 2 and 5 (Ad2 and Ad5)
possess a number of attributes which favor their use as gene delivery vehicles in gene …