Directed evolution: methodologies and applications
Directed evolution aims to expedite the natural evolution process of biological molecules
and systems in a test tube through iterative rounds of gene diversifications and library …
and systems in a test tube through iterative rounds of gene diversifications and library …
Meta-analysis of leukocyte diversity in atherosclerotic mouse aortas
The diverse leukocyte infiltrate in atherosclerotic mouse aortas was recently analyzed in 9
single-cell RNA sequencing and 2 mass cytometry studies. In a comprehensive meta …
single-cell RNA sequencing and 2 mass cytometry studies. In a comprehensive meta …
High-speed fluorescence image–enabled cell sorting
Fast and selective isolation of single cells with unique spatial and morphological traits
remains a technical challenge. Here, we address this by establishing high-speed image …
remains a technical challenge. Here, we address this by establishing high-speed image …
[HTML][HTML] Marked T cell activation, senescence, exhaustion and skewing towards TH17 in patients with COVID-19 pneumonia
S De Biasi, M Meschiari, L Gibellini, C Bellinazzi… - Nature …, 2020 - nature.com
The immune system of patients infected by SARS-CoV-2 is severely impaired. Detailed
investigation of T cells and cytokine production in patients affected by COVID-19 pneumonia …
investigation of T cells and cytokine production in patients affected by COVID-19 pneumonia …
[HTML][HTML] Impaired immune cell cytotoxicity in severe COVID-19 is IL-6 dependent
BACKGROUND Coronavirus disease 19 (COVID-19) is an emerging infectious disease
caused by SARS-CoV-2. Antiviral immune response is crucial to achieve pathogen …
caused by SARS-CoV-2. Antiviral immune response is crucial to achieve pathogen …
[HTML][HTML] Clonal expansion and epigenetic inheritance of long-lasting NK cell memory
Clonal expansion of cells with somatically diversified receptors and their long-term
maintenance as memory cells is a hallmark of adaptive immunity. Here, we studied …
maintenance as memory cells is a hallmark of adaptive immunity. Here, we studied …
[PDF][PDF] Precise reconstruction of the TME using bulk RNA-seq and a machine learning algorithm trained on artificial transcriptomes
A Zaitsev, M Chelushkin, D Dyikanov, I Cheremushkin… - Cancer Cell, 2022 - cell.com
Cellular deconvolution algorithms virtually reconstruct tissue composition by analyzing the
gene expression of complex tissues. We present the decision tree machine learning …
gene expression of complex tissues. We present the decision tree machine learning …
Guidelines for the use of flow cytometry and cell sorting in immunological studies
A Cossarizza, HD Chang, A Radbruch… - European journal of …, 2019 - Wiley Online Library
These guidelines are a consensus work of a considerable number of members of the
immunology and flow cytometry community. They provide the theory and key practical …
immunology and flow cytometry community. They provide the theory and key practical …
[HTML][HTML] Immunological signature in human cases of monkeypox infection in 2022 outbreak: an observational study
Background An unprecedented global monkeypox outbreak started in May, 2022. No data
are yet available about the dynamics of the immune response against monkeypox virus. The …
are yet available about the dynamics of the immune response against monkeypox virus. The …
[HTML][HTML] SARS-CoV-2–associated ssRNAs activate inflammation and immunity via TLR7/8
V Salvi, HO Nguyen, F Sozio, T Schioppa, C Gaudenzi… - JCI insight, 2021 - ncbi.nlm.nih.gov
The inflammatory and IFN pathways of innate immunity play a key role in the resistance and
pathogenesis of coronavirus disease 2019 (COVID-19). Innate sensors and SARS-CoV-2 …
pathogenesis of coronavirus disease 2019 (COVID-19). Innate sensors and SARS-CoV-2 …