Methodological guidelines to study extracellular vesicles

FAW Coumans, AR Brisson, EI Buzas… - Circulation …, 2017 - Am Heart Assoc
Owing to the relationship between extracellular vesicles (EVs) and physiological and
pathological conditions, the interest in EVs is exponentially growing. EVs hold high hopes …

Extracellular vesicles as biomarkers in liver diseases: a clinician's point of view

S Thietart, PE Rautou - Journal of hepatology, 2020 - Elsevier
Extracellular vesicles are membrane-bound vesicles containing proteins, lipids, RNAs and
microRNAs. They can originate from both healthy and stressed cells, and provide a …

Minimal information for studies of extracellular vesicles (MISEV2023): From basic to advanced approaches

JA Welsh, DCI Goberdhan, L O'Driscoll… - Journal of …, 2024 - Wiley Online Library
Extracellular vesicles (EVs), through their complex cargo, can reflect the state of their cell of
origin and change the functions and phenotypes of other cells. These features indicate …

Formation of a protein corona on the surface of extracellular vesicles in blood plasma

EÁ Tóth, L Turiák, T Visnovitz, C Cserép… - Journal of …, 2021 - Wiley Online Library
In this study we tested whether a protein corona is formed around extracellular vesicles
(EVs) in blood plasma. We isolated medium‐sized nascent EVs of THP1 cells as well as of …

Tetraspanins distinguish separate extracellular vesicle subpopulations in human serum and plasma–Contributions of platelet extracellular vesicles in plasma samples

N Karimi, R Dalirfardouei, T Dias… - Journal of …, 2022 - Wiley Online Library
Background: The ability to isolate extracellular vesicles (EVs) from blood is vital in the
development of EVs as disease biomarkers. Both serum and plasma can be used, but few …

Extracellular vesicles from human plasma and serum are carriers of extravesicular cargo—Implications for biomarker discovery

M Palviainen, M Saraswat, Z Varga, D Kitka… - PloS one, 2020 - journals.plos.org
Extracellular vesicles (EVs) in human blood are a potential source of biomarkers. To which
extent anticoagulation affects their concentration, cellular origin and protein composition is …

Minimal information for studies of extracellular vesicles 2018 (MISEV2018): a position statement of the International Society for Extracellular Vesicles and update of the …

C Théry, KW Witwer, E Aikawa… - Journal of …, 2018 - Wiley Online Library
The last decade has seen a sharp increase in the number of scientific publications
describing physiological and pathological functions of extracellular vesicles (EVs), a …

Obstacles and opportunities in the functional analysis of extracellular vesicle RNA–an ISEV position paper

B Mateescu, EJK Kowal, BWM Van Balkom… - Journal of …, 2017 - Taylor & Francis
The release of RNA-containing extracellular vesicles (EV) into the extracellular milieu has
been demonstrated in a multitude of different in vitro cell systems and in a variety of body …

Size-Exclusion Chromatography-based isolation minimally alters Extracellular Vesicles' characteristics compared to precipitating agents

A Gámez-Valero, M Monguió-Tortajada… - Scientific reports, 2016 - nature.com
Extracellular vesicles (EVs) have become an attractive field among the scientific community.
Yet, a major challenge is to define a consensus method for EVs isolation. Ultracentrifugation …

Isolation of exosomes from blood plasma: qualitative and quantitative comparison of ultracentrifugation and size exclusion chromatography methods

T Baranyai, K Herczeg, Z Onódi, I Voszka, K Módos… - PloS one, 2015 - journals.plos.org
Background Exosomes are emerging targets for biomedical research. However, suitable
methods for the isolation of blood plasma-derived exosomes without impurities have not yet …