The production of high yields of soluble recombinant protein is one of the main objectives of
protein biotechnology. Several factors, such as expression system, vector, host, media …

HIV-1 protease expression in the laboratory is demanding because of its high cytotoxicity,
making it difficult to express in bacterial expression systems such as Escherichia coli. To …

Rapid and high-throughput protein purification methods are required to explore structure
and function of several uncharacterized proteins. Isolation of recombinant protein expressed …

Polymerase chain reaction (PCR) is an efficient and one of the most common methods used
in biological sciences for in vitro multiplication of a target DNA molecule. The technique has …

Cloning and expression of a desired gene is indispensable in molecular biology studies.
Expression vectors, in this regard, should offer much needed flexibility and choice of cloning …

Transmembrane β-barrel proteins (OMPs) are highly robust structures for engineering and
development of nanopore channels, surface biosensors, and display libraries. Expanding …

The ability of histidine to participate in a wide range of stabilizing polar interactions
preferentially populates this residue in functionally important sites of proteins. Histidine …

We introduce OLIVAR (O rientation se L ection of I nsert in V ector through A ntisense R
eporter) as a novel selection strategy for the insertion of protein-coding genes into vector …

A natureza aniquiladora da ordem neoliberal capitalista atravessa diferentes domínios do
conhecimento e da vida. Ao percorrermos dois eixos analíticos distintos-biotecnologia e …

Chitinase is mostly investigated in Serratia marcescens, but not in S. plymuthica. In order to
detail investigated chitinase from S. plymuthica, a putative chitinase II fragment has been …