Environmental DNA/RNA for pathogen and parasite detection, surveillance, and ecology

D Bass, KW Christison, GD Stentiford, LSJ Cook… - Trends in …, 2023 - cell.com
Detection of pathogens, parasites, and other symbionts in environmental samples via
eDNA/eRNA (collectively eNA) is an increasingly important source of information about their …

Environmental DNA methods for ecological monitoring and biodiversity assessment in estuaries

RP Nagarajan, M Bedwell, AE Holmes, T Sanches… - Estuaries and …, 2022 - Springer
Environmental DNA (eDNA) detection methods can complement traditional biomonitoring to
yield new ecological insights in aquatic systems. However, the conceptual and …

Applications of the versatile CRISPR‐Cas13 RNA targeting system

M Kordyś, R Sen, Z Warkocki - Wiley Interdisciplinary Reviews …, 2022 - Wiley Online Library
CRISPR‐Cas are adaptable natural prokaryotic defense systems that act against invading
viruses and plasmids. Among the six currently known major CRISPR‐Cas types, the type VI …

The promise and pitfalls of environmental DNA and RNA approaches for the monitoring of human and animal pathogens from aquatic sources

JA Farrell, L Whitmore, DJ Duffy - Bioscience, 2021 - academic.oup.com
Novel forensics-inspired molecular approaches have revolutionized species detection in the
wild and are particularly useful for tracing endangered or invasive species. These new …

Seeing through sedimented waters: environmental DNA reduces the phantom diversity of sharks and rays in turbid marine habitats

YCA Ip, JJM Chang, KKP Lim, Z Jaafar… - BMC Ecology and …, 2021 - Springer
Background Sharks and rays are some of the most threatened marine taxa due to the high
levels of bycatch and significant demand for meat and fin-related products in many Asian …

Rapid CRISPR‐Cas13a genetic identification enables new opportunities for listed Chinook salmon management

MR Baerwald, EC Funk, AM Goodbla… - Molecular Ecology …, 2023 - Wiley Online Library
Accurate taxonomic identification is foundational for effective species monitoring and
management. When visual identifications are infeasible or inaccurate, genetic approaches …

Novel CRISPR/Cas technology in the realm of algal bloom biomonitoring: Recent trends and future perspectives

P Pal, U Anand, SC Saha, S Sundaramurthy… - Environmental …, 2023 - Elsevier
In conjunction with global climate change, progressive ocean warming, and acclivity in
pollution and anthropogenic eutrophication, the incidence of harmful algal blooms (HABs) …

CRISPR/Cas9-based lateral flow and fluorescence diagnostics

MJ Osborn, A Bhardwaj, SP Bingea, F Knipping… - Bioengineering, 2021 - mdpi.com
Clustered regularly interspaced short palindromic repeat (CRISPR/Cas) proteins can be
designed to bind specified DNA and RNA sequences and hold great promise for the …

Sensitive detection of foodborne pathogens based on CRISPR‐Cas13a

S Gao, J Liu, Z Li, Y Ma, J Wang - Journal of Food Science, 2021 - Wiley Online Library
Salmonella, being one of the most widespread foodborne pathogens, is a compulsory test
item required by national food safety standard of China and many other countries. More …

Potential applications of CRISPR/Cas for next-generation biomonitoring of harmful algae blooms: A review

B Durán-Vinet, K Araya-Castro, TC Chao, SA Wood… - Harmful algae, 2021 - Elsevier
Research on harmful algal and cyanobacterial blooms (HABs and CHABs) has risen
dramatically due to their increasing global distribution, frequency, and intensity. These …