[HTML][HTML] High-content CRISPR screening
CRISPR screens are a powerful source of biological discovery, enabling the unbiased
interrogation of gene function in a wide range of applications and species. In pooled …
interrogation of gene function in a wide range of applications and species. In pooled …
CRISPR-Cas9 system: A new-fangled dawn in gene editing
Till date, only three techniques namely Zinc Finger Nuclease (ZFN), Transcription-Activator
Like Effector Nucleases (TALEN) and Clustered Regularly Interspaced Short Palindromic …
Like Effector Nucleases (TALEN) and Clustered Regularly Interspaced Short Palindromic …
A comprehensive subcellular atlas of the Toxoplasma proteome via hyperLOPIT provides spatial context for protein functions
Apicomplexan parasites cause major human disease and food insecurity. They owe their
considerable success to highly specialized cell compartments and structures. These …
considerable success to highly specialized cell compartments and structures. These …
Uncovering the essential genes of the human malaria parasite Plasmodium falciparum by saturation mutagenesis
INTRODUCTION Malaria remains a devastating global parasitic disease, with the majority of
malaria deaths caused by the highly virulent Plasmodium falciparum. The extreme AT-bias …
malaria deaths caused by the highly virulent Plasmodium falciparum. The extreme AT-bias …
[HTML][HTML] Functional profiling of a Plasmodium genome reveals an abundance of essential genes
The genomes of malaria parasites contain many genes of unknown function. To assist drug
development through the identification of essential genes and pathways, we have measured …
development through the identification of essential genes and pathways, we have measured …
Cornerstones of CRISPR–Cas in drug discovery and therapy
C Fellmann, BG Gowen, PC Lin, JA Doudna… - Nature reviews Drug …, 2017 - nature.com
The recent development of CRISPR–Cas systems as easily accessible and programmable
tools for genome editing and regulation is spurring a revolution in biology. Paired with the …
tools for genome editing and regulation is spurring a revolution in biology. Paired with the …
Gliding motility powers invasion and egress in Apicomplexa
K Frénal, JF Dubremetz, M Lebrun… - Nature Reviews …, 2017 - nature.com
Protozoan parasites have developed elaborate motility systems that facilitate infection and
dissemination. For example, amoebae use actin-rich membrane extensions called …
dissemination. For example, amoebae use actin-rich membrane extensions called …
A CRISPR Cas9 high-throughput genome editing toolkit for kinetoplastids
T Beneke, R Madden, L Makin… - Royal Society …, 2017 - royalsocietypublishing.org
Clustered regularly interspaced short palindromic repeats (CRISPR), CRISPR-associated
gene 9 (Cas9) genome editing is set to revolutionize genetic manipulation of pathogens …
gene 9 (Cas9) genome editing is set to revolutionize genetic manipulation of pathogens …
Drugs in development for toxoplasmosis: advances, challenges, and current status
PH Alday, JS Doggett - Drug design, development and therapy, 2017 - Taylor & Francis
Toxoplasma gondii causes fatal and debilitating brain and eye diseases. Medicines that are
currently used to treat toxoplasmosis commonly have toxic side effects and require …
currently used to treat toxoplasmosis commonly have toxic side effects and require …
Identification of a master regulator of differentiation in Toxoplasma
Toxoplasma gondii chronically infects a quarter of the world's population, and its
recrudescence can cause life-threatening disease in immunocompromised individuals and …
recrudescence can cause life-threatening disease in immunocompromised individuals and …