The discovery of highly efficient gene targeting in Dictyostelium (De Lozanne and Spudich,
1987) and the use of the antisense RNA approach in this organism (Knecht and Loomis …

The phenomenon of homologous recombination, which allows specific gene conversion and
gene insertion, can be a powerful system for the study of eukaryotic cell biology. Data are …

Single-molecule imaging techniques were used to reveal the binding of individual cyclic
adenosine 3′, 5′-monophosphate molecules to heterotrimeric guanine nucleotide …

A computer method for detecting kinks and flexible sites in thread-like molecules was
developed. The method is based on the determination of the local curvature along the …

Coronin is an actin-binding protein in Dictyostelium discoideum that is enriched at the
leading edge of the cells and in projections of the cell surface called crowns. The …

Dictyostelium expresses 12 different myosins, including seven single-headed myosins I and
one conventional two-headed myosin II. In this review we focus on the signaling pathways …

Cortexillins I and II of D. discoideum constitute a novel subfamily of proteins with actin-
binding sites of the α-actinin/spectrin type. The C-terminal halves of these dimeric proteins …

To study In vivo functions of the ublqultous actlnbinding protein profilin, we generated by
antlsense and gene disruption techniques Dlctyostelium mutants that lack one or both of the …

Mitosis was studied in multinucleated and mononucleated mutant cells of Dictyostelium
discoideum that lack myosin II (EMBO J. 8, 923-932). Multinucleated cells were produced by …

We generated Dictyostelium double mutants lacking the two F-actin crosslinking proteins α-
actinin and gelation factor by inactivating the corresponding genes via homologous …