Advances in genome editing technology have made it possible to create genome-edited
(GE) animals, which are useful for identifying isolated genes and producing models of …

Transgenic (Tg) animal technology is one of the growing areas in biology. Various Tg
technologies, each with its own advantages and disadvantages, are available for generating …

Metal–organic frameworks (MOFs) are porous materials with unique characteristics that
make them well-suited for drug delivery and gene therapy applications. Among the MOFs …

The objectives were to investigate whether: 1) nanotransfectants are more effective than
other common transfection methods for SMGT; 2) NanoSMGT is able to transmit exogenous …

The objective was to introduce exogenous DNA into commercially sex-sorted bovine sperm
using nanopolymer for transfection. In the first experiment, the optimal concentration and …

“Sperm mediated gene transfer”(SMGT) is based on the ability of sperm cells to bind
exogenous DNA. The main objective of this study was to improve the production of …

A suggested alternative to improve post-thawed ram semen quality is the addition of seminal
plasma (SP). This is thought to be capable of improving sperm resistance to thermal shock …

The transgenic technologies represent potent biotechnological tools that allow the
generation of genetically modified animals useful for basic research and for biomedical …

Sperm-mediated gene transfer (SMGT) is based on the capacity of sperm to bind exogenous
DNA and transfer it into the oocyte during fertilization. In bovines, the progress of this …

Background Generating targeted mutant mice is a crucial technology in biomedical
research. This study focuses on optimizing the CRISPR/Cas9 system uptake into sperm cells …