Malaria parasitemia diagnosis and grading is hard and still far from perfection. Inaccurate
diagnosis and grading has caused tremendous deaths rate particularly in young children …

Introduction: High-resolution microscopy using fluorescent probes is a powerful tool to
investigate individual cell structure and function, cell subpopulations and mechanisms …

Nucleus segmentation is a challenging task due to the crowded distribution and blurry
boundaries of nuclei. Recent approaches represent nuclei by means of polygons to …

A prerequisite for the systems biology analysis of tissues is an accurate digital three-
dimensional reconstruction of tissue structure based on images of markers covering multiple …

Three-dimensional multicellular aggregates such as spheroids provide reliable in vitro
substitutes for tissues. Quantitative characterization of spheroids at the cellular level is …

Background Due to the large amount of data produced by advanced microscopy, automated
image analysis is crucial in modern biology. Most applications require reliable cell nuclei …

During mammalian preimplantation, cells of the inner cell mass (ICM) adopt either an
embryonic or an extraembryonic fate. This process is tightly regulated in space and time and …

In many situations, 3D cell cultures mimic the natural organization of tissues more closely
than 2D cultures. Conventional methods for phenotyping such 3D cultures use either single …

Replicating the naturalistic biomechanical milieu of cells is a primary requisite to uncover the
fundamental life processes. The native milieu is significantly not replicated in the two …

One of the most important and error-prone tasks in biological image analysis is the
segmentation of touching or overlapping cells. Particularly for optical microscopy, including …