In-cell structural biology by NMR: the benefits of the atomic scale
FX Theillet - Chemical reviews, 2022 - ACS Publications
In-cell structural biology aims at extracting structural information about proteins or nucleic
acids in their native, cellular environment. This emerging field holds great promise and is …
acids in their native, cellular environment. This emerging field holds great promise and is …
Nanobodies as molecular imaging probes
Camelidae derived single-domain antibodies (sdAbs), commonly known as nanobodies
(Nbs), are the smallest antibody fragments with full antigen-binding capacity. Owing to their …
(Nbs), are the smallest antibody fragments with full antigen-binding capacity. Owing to their …
BUB1 and CENP-U, primed by CDK1, are the main PLK1 kinetochore receptors in mitosis
Reflecting its pleiotropic functions, Polo-like kinase 1 (PLK1) localizes to various sub-cellular
structures during mitosis. At kinetochores, PLK1 contributes to microtubule attachments and …
structures during mitosis. At kinetochores, PLK1 contributes to microtubule attachments and …
RZZ‐Spindly and CENP‐E form an integrated platform to recruit dynein to the kinetochore corona
Chromosome biorientation on the mitotic spindle is prerequisite to errorless genome
inheritance. CENP‐E (kinesin‐7) and dynein–dynactin (DD), microtubule motors with …
inheritance. CENP‐E (kinesin‐7) and dynein–dynactin (DD), microtubule motors with …
Conformational transitions of the Spindly adaptor underlie its interaction with Dynein and Dynactin
Cytoplasmic Dynein 1, or Dynein, is a microtubule minus end–directed motor. Dynein
motility requires Dynactin and a family of activating adaptors that stabilize the Dynein …
motility requires Dynactin and a family of activating adaptors that stabilize the Dynein …
Assembly principles and stoichiometry of a complete human kinetochore module
K Walstein, A Petrovic, D Pan, B Hagemeier, D Vogt… - Science …, 2021 - science.org
Centromeres are epigenetically determined chromosomal loci that seed kinetochore
assembly to promote chromosome segregation during cell division. CENP-A, a centromere …
assembly to promote chromosome segregation during cell division. CENP-A, a centromere …
Structure of the human KMN complex and implications for regulation of its assembly
S Polley, T Raisch, S Ghetti, M Körner… - Nature structural & …, 2024 - nature.com
Biorientation of chromosomes during cell division is necessary for precise dispatching of a
mother cell's chromosomes into its two daughters. Kinetochores, large layered structures …
mother cell's chromosomes into its two daughters. Kinetochores, large layered structures …
In-cell double electron–electron resonance at nanomolar protein concentrations
S Kucher, C Elsner, M Safonova, S Maffini… - The Journal of …, 2021 - ACS Publications
Electron paramagnetic resonance (EPR) spectroscopy is an established technique to site-
specifically monitor conformational changes of spin-labeled biomolecules. Emerging in-cell …
specifically monitor conformational changes of spin-labeled biomolecules. Emerging in-cell …
Structure of the RZZ complex and molecular basis of Spindly‐driven corona assembly at human kinetochores
In metazoans, a≈ 1 megadalton (MDa) multiprotein complex comprising the dynein–
dynactin adaptor Spindly and the ROD–Zwilch–ZW10 (RZZ) complex is the building block of …
dynactin adaptor Spindly and the ROD–Zwilch–ZW10 (RZZ) complex is the building block of …
Altered conformation of α-synuclein drives dysfunction of synaptic vesicles in a synaptosomal model of Parkinson's disease
While misfolding of alpha-synuclein (αSyn) is central to the pathogenesis of Parkinson's
disease (PD), fundamental questions about its structure and function at the synapse remain …
disease (PD), fundamental questions about its structure and function at the synapse remain …