Unraveling plant–microbe symbioses using single-cell and spatial transcriptomics

K Serrano, F Tedeschi, SU Andersen… - Trends in Plant Science, 2024 - cell.com
Plant–microbe symbioses require intense interaction and genetic coordination to
successfully establish in specific cell types of the host and symbiont. Traditional RNA-seq …

Lost in space: what single-cell RNA sequencing cannot tell you

K Adema, MA Schon, MD Nodine, W Kohlen - Trends in Plant Science, 2024 - cell.com
Plant scientists are rapidly integrating single-cell RNA sequencing (scRNA-seq) into their
workflows. Maximizing the potential of scRNA-seq requires a proper understanding of the …

[HTML][HTML] Cellular insights into legume root infection by rhizobia

F de Carvalho-Niebel, J Fournier, A Becker… - Current Opinion in Plant …, 2024 - Elsevier
Legume plants establish an endosymbiosis with nitrogen-fixing rhizobia bacteria, which are
taken up from the environment anew by each host generation. This requires a dedicated …

Advancing our understanding of root development: Technologies and insights from diverse studies

NS Coll, M Moreno-Risueno, LC Strader… - Plant …, 2024 - academic.oup.com
Understanding root development is critical for enhancing plant growth and health, and
advanced technologies are essential for unraveling the complexities of these processes. In …

Comparative genomics, pangenomics, and phenomic studies of Pectobacterium betavasculorum strains isolated from sugar beet, potato, sunflower, and artichoke …

M Borowska-Beszta, M Smoktunowicz… - Frontiers in Plant …, 2024 - frontiersin.org
Introduction Bacteria of genus Pectobacterium, encompassing economically significant
pathogens affecting various plants, includes the species P. betavasculorum, initially …

Laser Capture Microdissection Transcriptome Reveals Spatiotemporal Tissue Gene Expression Patterns of Medicago truncatula Roots Responding to Rhizobia

E Schnabel, J Thomas, R El-Hawaz… - Molecular Plant …, 2023 - Am Phytopath Society
We report a public resource for examining the spatiotemporal RNA expression of 54,893
Medicago truncatula genes during the first 72 h of response to rhizobial inoculation. Using a …

[HTML][HTML] Single-Cell Transcriptomics Applied in Plants

Y Sun, J Sun, C Lin, J Zhang, H Yan, Z Guan, C Zhang - Cells, 2024 - mdpi.com
Single-cell RNA sequencing (scRNA-seq) is a high-tech method for characterizing the
expression patterns of heterogeneous cells in the same tissue and has changed our …

The Defective in Autoregulation (DAR) gene of Medicago truncatula encodes a protein involved in regulating nodulation and arbuscular mycorrhiza

E Schnabel, S Bashyal, C Corbett, T Kassaw… - BMC plant biology, 2024 - Springer
Background Legumes utilize a long-distance signaling feedback pathway, termed
Autoregulation of Nodulation (AON), to regulate the establishment and maintenance of their …

[HTML][HTML] Cellular morphodynamics and signaling around the transcellular passage cleft during rhizobial infections of legume roots

G Zhang, T Ott - Current Opinion in Cell Biology, 2024 - Elsevier
Legume roots allow intracellular infections of rhizobia to establish the mutualistic root nodule
symbiosis. During this colonization event, specialized and membrane-defined infection …

TML1 and TML2 synergistically regulate nodulation and affect arbuscular mycorrhiza in Medicago truncatula

D Chaulagain, E Schnabel, M Kappes, EX Lin… - Frontiers in Plant …, 2024 - frontiersin.org
Two symbiotic processes, nodulation and arbuscular mycorrhiza, are primarily controlled by
the plant's need for nitrogen (N) and phosphorus (P), respectively. Autoregulation of …