Natural photoreceptors as a source of fluorescent proteins, biosensors, and optogenetic tools

DM Shcherbakova, AA Shemetov… - Annual review of …, 2015 - annualreviews.org
Genetically encoded optical tools have revolutionized modern biology by allowing detection
and control of biological processes with exceptional spatiotemporal precision and sensitivity …

Engineering of bacterial phytochromes for near-infrared imaging, sensing, and light-control in mammals

KD Piatkevich, FV Subach, VV Verkhusha - Chemical Society reviews, 2013 - pubs.rsc.org
Near-infrared light is favourable for imaging in mammalian tissues due to low absorbance of
hemoglobin, melanin, and water. Therefore, fluorescent proteins, biosensors and …

Near-infrared fluorescent proteins for multicolor in vivo imaging

DM Shcherbakova, VV Verkhusha - Nature methods, 2013 - nature.com
Near-infrared fluorescent proteins (FPs) are in high demand for in vivo imaging. We
developed four spectrally distinct near-infrared FPs—iRFP670, iRFP682, iRFP702 and …

Unraveling the Carrier Dynamics of BiVO4: A Femtosecond to Microsecond Transient Absorption Study

J Ravensbergen, FF Abdi, JH Van Santen… - The Journal of …, 2014 - ACS Publications
Bismuth vanadate (BiVO4) is a promising semiconductor material for photoelectrochemical
water splitting showing good visible light absorption and a high photochemical stability. To …

Spectroscopic and computational observation of glutamine tautomerization in the blue light sensing using flavin domain photoreaction

Y Hontani, J Mehlhorn, T Domratcheva… - Journal of the …, 2023 - ACS Publications
Blue light sensing using flavin (BLUF) domains constitute a family of flavin-binding
photoreceptors of bacteria and eukaryotic algae. BLUF photoactivation proceeds via a light …

Structure-guided engineering enhances a phytochrome-based infrared fluorescent protein

ME Auldridge, KA Satyshur, DM Anstrom… - Journal of Biological …, 2012 - ASBMB
Phytochrome is a multidomain dimeric red light photoreceptor that utilizes a chromophore-
binding domain (CBD), a PHY domain, and an output module to induce cellular changes in …

High-resolution crystal structures of transient intermediates in the phytochrome photocycle

M Carrillo, S Pandey, J Sanchez, M Noda, I Poudyal… - Structure, 2021 - cell.com
Phytochromes are red/far-red light photoreceptors in bacteria to plants, which elicit a variety
of important physiological responses. They display a reversible photocycle between the …

Time-resolved macromolecular crystallography at pulsed X-ray sources

M Schmidt - International journal of molecular sciences, 2019 - mdpi.com
The focus of structural biology is shifting from the determination of static structures to the
investigation of dynamical aspects of macromolecular function. With time-resolved …

Reaction dynamics of the chimeric channelrhodopsin C1C2

Y Hontani, M Marazzi, K Stehfest, T Mathes… - Scientific Reports, 2017 - nature.com
Channelrhodopsin (ChR) is a key protein of the optogenetic toolkit. C1C2, a functional
chimeric protein of Chlamydomonas reinhardtii ChR1 and ChR2, is the only ChR whose …

Origins of fluorescence in evolved bacteriophytochromes

S Bhattacharya, ME Auldridge, H Lehtivuori… - Journal of Biological …, 2014 - ASBMB
Use of fluorescent proteins to study in vivo processes in mammals requires near-infrared
(NIR) biomarkers that exploit the ability of light in this range to penetrate tissue …