After three decades of developments, single particle tracking (SPT) has become a powerful
tool to interrogate dynamics in a range of materials including live cells and novel catalytic …

One of the grand challenges of biophysical chemistry is to understand the principles that
govern protein misfolding and aggregation, which is a highly complex process that is …

Apical growth in plants initiates upon seed germination, whereas radial growth is primed
only during early ontogenesis in procambium cells and activated later by the vascular …

The phytohormone auxin plays crucial roles in nearly every aspect of plant growth and
development. The auxin response factor (ARF) transcription factor family regulates auxin …

Punishment is a popular tool when governing commons in situations where free riders
would otherwise take over. It is well known that sanctioning systems, such as the police and …

In recent years fluorescence correlation spectroscopy (FCS) has become a routine method
for determining diffusion coefficients, chemical rate constants, molecular concentrations …

Cell biologists strive to characterize molecular interactions directly in the intracellular
environment. The intrinsic resolution of optical microscopy, however, allows visualization of …

This protocol outlines a procedure for collecting and analyzing point spread functions
(PSFs). It describes how to prepare fluorescent microsphere samples, set up a confocal …

We have implemented scanning fluorescence correlation spectroscopy (sFCS) for precise
determination of diffusion coefficients of fluorescent molecules in solution. The measurement …

We describe a technique based on moment-analysis for the measurement of the average
number of molecules and brightness in each pixel in fluorescence microscopy images. The …