Cryogenic super-resolution fluorescence and electron microscopy correlated at the nanoscale

PD Dahlberg, WE Moerner - Annual Review of Physical …, 2021 - annualreviews.org
We review the emerging method of super-resolved cryogenic correlative light and electron
microscopy (srCryoCLEM). Super-resolution (SR) fluorescence microscopy and cryogenic …

Chip-based multimodal super-resolution microscopy for histological investigations of cryopreserved tissue sections

LE Villegas-Hernández, V Dubey, M Nystad… - Light: Science & …, 2022 - nature.com
Histology involves the observation of structural features in tissues using a microscope. While
diffraction-limited optical microscopes are commonly used in histological investigations, their …

High-contrast imaging of nanodiamonds in cells by energy filtered and correlative light-electron microscopy: toward a quantitative nanoparticle-cell analysis

S Han, M Raabe, L Hodgson, J Mantell, P Verkade… - Nano Letters, 2019 - ACS Publications
Fluorescent nanodiamonds (fNDs) represent an emerging class of nanomaterials offering
great opportunities for ultrahigh resolution imaging, sensing and drug delivery applications …

Studying the morphology, composition and function of the photoreceptor primary cilium in zebrafish

M Masek, J Zang, JM Mateos, M Garbelli, U Ziegler… - Methods in Cell …, 2023 - Elsevier
Vision is one of our dominant senses and its loss has a profound impact on the life quality of
affected individuals. Highly specialized neurons in the retina called photoreceptors convert …

TEM, SEM, and STEM-based immuno-CLEM workflows offer complementary advantages

V Oorschot, BW Lindsey, J Kaslin, G Ramm - Scientific Reports, 2021 - nature.com
Identifying endogenous tissue stem cells remains a key challenge in developmental and
regenerative biology. To distinguish and molecularly characterise stem cell populations in …

[HTML][HTML] Loss-of-function of the ciliopathy protein Cc2d2a disorganizes the vesicle fusion machinery at the periciliary membrane and indirectly affects Rab8-trafficking …

I Ojeda Naharros, M Gesemann, JM Mateos… - PLoS …, 2017 - journals.plos.org
Ciliopathies are human disorders caused by dysfunction of primary cilia, ubiquitous
organelles involved in transduction of environmental signals such as light sensation in …

Correlative imaging of biological tissues with apertureless scanning near-field optical microscopy and confocal laser scanning microscopy

SG Stanciu, DE Tranca, R Hristu… - Biomedical optics …, 2017 - opg.optica.org
Apertureless scanning near-field optical microscopy (ASNOM) has attracted considerable
interest over the past years as a result of its valuable contrast mechanisms and capabilities …

Direct imaging of uncoated biological samples enables correlation of super-resolution and electron microscopy data

JM Mateos, G Barmettler, J Doehner, A Kaech… - Scientific reports, 2018 - nature.com
A simple method for imaging biological tissue samples by electron microscopy and its
correlation with super-resolution light microscopy is presented. This room temperature …

A new zebrafish model for CACNA2D4-dysfunction

DK Schlegel, SMK Glasauer, JM Mateos… - … & visual science, 2019 - iovs.arvojournals.org
Purpose: Mutations in CACNA2D4, encoding the α 2 δ 4 subunit of retinal voltage-gated
calcium channels (Ca v), cause a rare type of retinal dysfunction in human, mainly affecting …

Chip-based multimodal super-resolution microscopy for histological investigations of cryopreserved tissue sections

L Villegas, VK Dubey, M Nystad, JC Tinguely… - … -Hernández, LE (2023) …, 2022 - munin.uit.no
Histology involves the observation of structural features in tissues using a microscope. While
diffraction-limited optical microscopes are commonly used in histological investigations, their …