Mouse embryonic stem (ES) cells perpetuate in vitro the broad developmental potential of
naïve founder cells in the preimplantation embryo. ES cells self-renew relentlessly in culture …

Pluripotent stem cells constitute a platform to model disease and developmental processes
and can potentially be used in regenerative medicine. However, not all pluripotent cell lines …

It has long been the dream of biologists to map gene expression at the single-cell level. With
such data one might track heterogeneous cell sub-populations, and infer regulatory …

Embryonic stem cell (ESC) culture conditions are important for maintaining long-term self-
renewal, and they influence cellular pluripotency state. Here, we report single cell RNA …

Pluripotent stem cells (PSCs) are capable of dynamic interconversion between distinct
substates; however, the regulatory circuits specifying these states and enabling transitions …

The inheritance of epigenetic marks, in particular DNA methylation, provides a molecular
memory that ensures faithful commitment to transcriptional programs during mammalian …

Pluripotent stem cells can self-renew in culture and differentiate along all somatic lineages
in vivo. While much is known about the molecular basis of pluripotency, the mechanisms of …

When pluripotent cells are exposed to a uniform culture environment they routinely display
heterogeneous gene expression. Aspects of this heterogeneity, such as Nanog expression …

Differentiating somatic cells are progressively restricted to specialized functions during
ontogeny, but they can be experimentally directed to form other cell types, including those …

Embryonic stem cells (ESCs) can be maintained in culture indefinitely while retaining the
capacity to generate any type of cell in the body, and therefore not only hold great promise …