Targeted genome editing using engineered nucleases has rapidly gone from being a niche
technology to a mainstream method used by many biological researchers. This widespread …

Zinc-finger nucleases (ZFNs) and transcription activator-like effector nucleases (TALENs)
comprise a powerful class of tools that are redefining the boundaries of biological research …

Mitochondrial DNA double-strand breaks (mtDSBs) are toxic lesions that compromise the
integrity of mitochondrial DNA (mtDNA) and alter mitochondrial function. Communication …

Clustered, regularly interspaced, short palindromic repeat (CRISPR) RNA-guided nucleases
(RGNs) are highly efficient genome editing tools,,. CRISPR-associated 9 (Cas9) RGNs are …

RNA-guided engineered nucleases (RGENs) derived from the prokaryotic adaptive immune
system known as CRISPR (clustered, regularly interspaced, short palindromic repeat)/Cas …

Programmable nucleases—including zinc-finger nucleases (ZFNs), transcription activator-
like effector nucleases (TALENs) and RNA-guided engineered nucleases (RGENs) derived …

RNA-guided Cas9 nucleases derived from clustered regularly interspaced short palindromic
repeats (CRISPR)-Cas systems have dramatically transformed our ability to edit the …

We employ the CRISPR-Cas system of Streptococcus pyogenes as programmable RNA-
guided endonucleases (RGENs) to cleave DNA in a targeted manner for genome editing in …

Targeted nucleases have provided researchers with the ability to manipulate virtually any
genomic sequence, enabling the facile creation of isogenic cell lines and animal models for …

Engineered nucleases enable the targeted alteration of nearly any gene in a wide range of
cell types and organisms. The newly-developed transcription activator-like effector …