The culture of Chinese Hamster Ovary (CHO) cells for modern industrial applications, such
as expression of recombinant proteins, requires media that support growth and production …

At present, nearly 70% of recombinant therapeutic proteins (RTPs) are produced by Chinese
hamster ovary (CHO) cells, and serum-free medium (SFM) is necessary for their culture to …

Abstract Design of Experiments methods offer systematic tools for bioprocess development
in Quality by Design, but their major drawback is the user-defined choice of factor boundary …

An innovative, Raman spectroscopy‐based monitoring and control system is introduced in
this paper for designing dynamic feeding strategies that allow the maintenance of key …

Medium optimization is a crucial step during cell culture for biopharmaceutics and
regenerative medicine; however, this step remains challenging, as both media and cells are …

Raman spectroscopy is a multipurpose analytical technology that has found great utility in
real‐time monitoring and control of critical performance parameters of cell culture processes …

Process characterization is necessary in the biopharmaceutical industry, leading to concepts
such as design of experiments (DoE) in combination with process modeling. However, these …

The culture media optimization is an inevitable part of upstream process development in
therapeutic monoclonal antibodies (mAbs) production. The quality by design (QbD) …

The monitoring and control of bioprocesses is of the utmost importance in order to provide a
consistent, safe, and high‐quality product for consumers. Current monitoring and control …

A novel approach of design of experiment (DoE) is developed for the optimization of key
substrates of the culture medium, amino acids, and sugars, by utilizing perfusion …