Novel CRISPR–Cas systems: an updated review of the current achievements, applications, and future research perspectives

S Nidhi, U Anand, P Oleksak, P Tripathi, JA Lal… - International journal of …, 2021 - mdpi.com
According to Darwin's theory, endless evolution leads to a revolution. One such example is
the Clustered Regularly Interspaced Palindromic Repeats (CRISPR)–Cas system, an …

Alternative therapeutic strategies to treat antibiotic-resistant pathogens

CR MacNair, ST Rutherford, MW Tan - Nature Reviews Microbiology, 2024 - nature.com
Resistance threatens to render antibiotics—which are essential for modern medicine—
ineffective, thus posing a threat to human health. The discovery of novel classes of …

Engineered phage with antibacterial CRISPR–Cas selectively reduce E. coli burden in mice

YE Gencay, D Jasinskytė, C Robert, S Semsey… - Nature …, 2024 - nature.com
Antibiotic treatments have detrimental effects on the microbiome and lead to antibiotic
resistance. To develop a phage therapy against a diverse range of clinically relevant …

Comprehensive review of CRISPR-based gene editing: mechanisms, challenges, and applications in cancer therapy

M Chehelgerdi, M Chehelgerdi… - Molecular cancer, 2024 - Springer
The CRISPR system is a revolutionary genome editing tool that has the potential to
revolutionize the field of cancer research and therapy. The ability to precisely target and edit …

Advances in industrial biotechnology using CRISPR-Cas systems

PD Donohoue, R Barrangou, AP May - Trends in biotechnology, 2018 - cell.com
The term 'clustered regularly interspaced short palindromic repeats'(CRISPR) has recently
become synonymous with the genome-editing revolution. The RNA-guided endonuclease …

High‐efficiency delivery of CRISPR‐Cas9 by engineered probiotics enables precise microbiome editing

K Neil, N Allard, P Roy, F Grenier… - Molecular systems …, 2021 - embopress.org
Antibiotic resistance threatens our ability to treat infectious diseases, spurring interest in
alternative antimicrobial technologies. The use of bacterial conjugation to deliver CRISPR …

[HTML][HTML] Engineering therapeutic phages for enhanced antibacterial efficacy

S Meile, J Du, M Dunne, S Kilcher… - Current opinion in virology, 2022 - Elsevier
The alarming rise in antimicrobial resistance coupled with a lack of innovation in antibiotics
has renewed interest in the development of alternative therapies to combat bacterial …

Conjugative delivery of CRISPR-Cas9 for the selective depletion of antibiotic-resistant enterococci

M Rodrigues, SW McBride, K Hullahalli… - Antimicrobial Agents …, 2019 - Am Soc Microbiol
The innovation of new therapies to combat multidrug-resistant (MDR) bacteria is being
outpaced by the continued rise of MDR bacterial infections. Of particular concern are …

Crispr-cas: Converting a bacterial defence mechanism into a state-of-the-art genetic manipulation tool

A Loureiro, GJ da Silva - Antibiotics, 2019 - mdpi.com
Bacteriophages are pervasive viruses that infect bacteria, relying on their genetic machinery
to replicate. In order to protect themselves from this kind of invader, bacteria developed an …

[HTML][HTML] Theragnostic application of nanoparticle and CRISPR against food-borne multi-drug resistant pathogens

R Bhattacharjee, A Nandi, P Mitra, K Saha, P Patel… - Materials Today Bio, 2022 - Elsevier
Foodborne infection is one of the leading sources of infections spreading across the world.
Foodborne pathogens are recognized as multidrug-resistant (MDR) pathogens posing a …