Circular RNAs (circRNAs) are a class of non-coding RNAs (ncRNAs) that are involved in
transcriptional and posttranscriptional gene expression regulation. The development of …

In the few years since its initial application, massively parallel cDNA sequencing, or RNA-
seq, has allowed many advances in the characterization and quantification of …

Through alternative splicing, most human genes express multiple isoforms that often differ in
function. To infer isoform regulation from high-throughput sequencing of cDNA fragments …

TopHat-Fusion is an algorithm designed to discover transcripts representing fusion gene
products, which result from the breakage and re-joining of two different chromosomes, or …

Abstract We describe Trans-ABySS, a de novo short-read transcriptome assembly and
analysis pipeline that addresses variation in local read densities by assembling read …

Next-generation sequencing technologies have revolutionarily advanced sequence-based
research with the advantages of high-throughput, high-sensitivity, and high-speed. RNA-seq …

From RNA-seq reads to differential expression results | Genome Biology Skip to main content
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Advances in genome sequencing have progressed at a rapid pace, with increased
throughput accompanied by plunging costs. But these advances go far beyond faster and …

Transcriptome sequencing allows for analysis of mature RNAs at base pair resolution. Here
we show that RNA-seq can also be used for studying nascent RNAs undergoing …

While the majority of multiexonic human genes show some evidence of alternative splicing,
it is unclear what fraction of observed splice forms is functionally relevant. In this study, we …