The Mediator complex, which in humans is 1.4 MDa in size and includes 26 subunits,
controls many aspects of RNA polymerase II (Pol II) function. Apart from its size, a defining …

Abstract The RNA polymerase II (Pol II) pre-initiation complex (PIC) is a critical node in
eukaryotic transcription regulation, and its formation is the major rate-limiting step in …

Alterations in the regulation of gene expression are frequently associated with
developmental diseases or cancer. Transcription activation is a key phenomenon in the …

All multicellular organisms rely on differential gene transcription regulated by genomic
enhancers, which function through cofactors that are recruited by transcription factors …

Nuclear pore complexes play central roles as gatekeepers of RNA and protein transport
between the cytoplasm and nucleoplasm. However, their large size and dynamic nature …

Eukaryotic transcription requires the assembly of a multisubunit preinitiation complex (PIC)
composed of RNA polymerase II (Pol II) and the general transcription factors. The coactivator …

INTRODUCTION The multisubunit Mediator binds RNA polymerase II (Pol II), transduces
regulatory signals from transcription factors to Pol II, facilitates preinitiation complex (PIC) …

Abstract RNA polymerase II (Pol II) transcribes all protein-coding genes and many
noncoding RNAs in eukaryotic genomes. Although Pol II is a complex, 12-subunit enzyme, it …

Abstract The RNA polymerase II (Pol II) enzyme transcribes all protein-coding and most non-
coding RNA genes and is globally regulated by Mediator—a large, conformationally flexible …

While Mediator plays a key role in eukaryotic transcription, little is known about its
mechanism of action. This study combines CRISPR-Cas9 genetic screens, degron assays …