Microbial production of limonene and its derivatives: Achievements and perspectives

Y Ren, S Liu, G Jin, X Yang, YJ Zhou - Biotechnology Advances, 2020 - Elsevier
Limonene and its derivatives have great market potential with diverse applications in food,
pharmaceuticals, cosmetics, etc. Commercial production of limonene and its derivatives …

Guidelines for development and implementation of biocatalytic P450 processes

MT Lundemo, JM Woodley - Applied microbiology and biotechnology, 2015 - Springer
Biocatalytic reactions performed by cytochrome P450 monooxygenases are interesting in
pharmaceutical research since they are involved in human drug metabolism. Furthermore …

Pseudomonas putida KT2440 is HV1 certified, not GRAS

LFC Kampers, RJM Volkers… - Microbial …, 2019 - Wiley Online Library
Pseudomonas putida is rapidly becoming a workhorse for industrial production due to its
metabolic versatility, genetic accessibility and stress‐resistance properties. The P. putida …

Outer membrane protein AlkL boosts biocatalytic oxyfunctionalization of hydrophobic substrates in Escherichia coli

MK Julsing, M Schrewe, S Cornelissen… - Applied and …, 2012 - Am Soc Microbiol
The outer membrane of microbial cells forms an effective barrier for hydrophobic
compounds, potentially causing an uptake limitation for hydrophobic substrates. Low …

Synthesis of ω‐hydroxy dodecanoic acid based on an engineered CYP153A fusion construct

D Scheps, S Honda Malca, SM Richter… - Microbial …, 2013 - Wiley Online Library
A bacterial P 450 monooxygenase‐based whole cell biocatalyst using Escherichia coli has
been applied in the production of ω‐hydroxy dodecanoic acid from dodecanoic acid (C12 …

Whole‐cell‐based CYP153A6‐catalyzed (S)‐limonene hydroxylation efficiency depends on host background and profits from monoterpene uptake via AlkL

S Cornelissen, MK Julsing, J Volmer… - Biotechnology and …, 2013 - Wiley Online Library
Living microbial cells are considered to be the catalyst of choice for selective terpene
functionalization. However, such processes often suffer from side product formation and …

Continuous cyclohexane oxidation to cyclohexanol using a novel cytochrome P450 monooxygenase from Acidovorax sp. CHX100 in recombinant P. taiwanensis …

R Karande, L Debor, D Salamanca… - Biotechnology and …, 2016 - Wiley Online Library
The applications of biocatalysts in chemical industries are characterized by activity,
selectivity, and stability. One key strategy to achieve high biocatalytic activity is the …

Biocatalytic conversion of cycloalkanes to lactones using an in‐vivo cascade in Pseudomonas taiwanensis VLB120

R Karande, D Salamanca, A Schmid… - Biotechnology and …, 2018 - Wiley Online Library
Chemical synthesis of lactones from cycloalkanes is a multi‐step process challenged by
limitations in reaction efficiency (conversion and yield), atom economy (by‐products) and …

Maximizing Biocatalytic Cyclohexane Hydroxylation by Modulating Cytochrome P450 Monooxygenase Expression in P. taiwanensis VLB120

L Schäfer, R Karande, B Bühler - Frontiers in Bioengineering and …, 2020 - frontiersin.org
Cytochrome P450 monooxygenases (Cyps) effectively catalyze the regiospecific
oxyfunctionalization of inert C–H bonds under mild conditions. Due to their cofactor …

Whole-cell hydroxylation of n-octane by Escherichia coli strains expressing the CYP153A6 operon

RK Gudiminchi, C Randall, DJ Opperman… - Applied microbiology …, 2012 - Springer
CYP153A6 is a well-studied terminal alkane hydroxylase which has previously been
expressed in Pseudomonas putida and Escherichia coli by using the pCom8 plasmid. In this …