To elucidate principles operating in native biological systems and to develop novel
biotechnologies, synthetic biology aims to build and integrate synthetic gene circuits within …

Transcriptome engineering applications in living cells with RNA-targeting CRISPR effectors
depend on accurate prediction of on-target activity and off-target avoidance. Here we design …

Controlling access to genomic information and maintaining its stability are key aspects of
cell life. Histone acetylation is a reversible epigenetic modification that allows access to DNA …

Pooled CRISPR screens coupled with single-cell RNA-sequencing have enabled systematic
interrogation of gene function and regulatory networks. Here, we introduce Cas13 RNA …

Despite ground-breaking genetic studies that have identified thousands of risk variants for
developmental diseases, how these variants lead to molecular and cellular phenotypes …

Histone H3. 3 is frequently mutated in tumors, with the lysine 36 to methionine mutation
(K36M) being a hallmark of chondroblastomas. While it is known that H3. 3K36M changes …

Tight control of the transcription process is essential for the correct spatial and temporal
gene expression pattern during development and in homeostasis. Enhancers are at the core …

Complex transcriptional control is a conserved feature of both eukaryotes and the viruses
that infect them. Despite viral genomes being smaller and more gene dense than their hosts …

A key question in regulatory genomics is whether cis-regulatory elements (CREs) are
modular elements that can function anywhere in the genome, or whether they are adapted to …

The epigenome, comprising DNA and histone modifications alongside intricate chromatin
structures, has emerged as pivotal players in disease development. These factors offer …