Schrödinger's microbes: tools for distinguishing the living from the dead in microbial ecosystems

JB Emerson, RI Adams, CMB Román, B Brooks… - Microbiome, 2017 - Springer
While often obvious for macroscopic organisms, determining whether a microbe is dead or
alive is fraught with complications. Fields such as microbial ecology, environmental health …

Advances in rapid detection methods for foodborne pathogens

X Zhao, CW Lin, J Wang, DH Oh - Journal of microbiology and …, 2014 - koreascience.kr
Food safety is increasingly becoming an important public health issue, as foodborne
diseases present a widespread and growing public health problem in both developed and …

Presence and Persistence of Salmonella in Water: The Impact on Microbial Quality of Water and Food Safety

H Liu, CA Whitehouse, B Li - Frontiers in Public Health, 2018 - frontiersin.org
Salmonella ranks high among the pathogens causing foodborne disease outbreaks.
According to the Centers for Disease Control and Prevention, Salmonella contributed to …

Dead or alive: molecular assessment of microbial viability

GA Cangelosi, JS Meschke - Applied and environmental …, 2014 - Am Soc Microbiol
Nucleic acid-based analytical methods, ranging from species-targeted PCRs to
metagenomics, have greatly expanded our understanding of microbiological diversity in …

Culture‐independent rapid detection methods for bacterial pathogens and toxins in food matrices

Y Wang, JK Salazar - … Reviews in Food Science and Food …, 2016 - Wiley Online Library
Rapid detection of bacterial pathogens and toxins in foods is necessary to provide real‐time
results to mitigate foodborne illness outbreaks. Cultural enrichment methods, although the …

Next generation sequencing approaches to evaluate water and wastewater quality

E Garner, BC Davis, E Milligan, MF Blair, I Keenum… - Water Research, 2021 - Elsevier
The emergence of next generation sequencing (NGS) is revolutionizing the potential to
address complex microbiological challenges in the water industry. NGS technologies can …

Progress in understanding preferential detection of live cells using viability dyes in combination with DNA amplification

M Fittipaldi, A Nocker, F Codony - Journal of microbiological methods, 2012 - Elsevier
The ideal scenario in most applications of microbial diagnostics is that only viable cells are
detected. Bacteria were traditionally considered viable when they could be cultured …

Point-of-care DNA amplification for disease diagnosis and management

JR Botella - Annual Review of Phytopathology, 2022 - annualreviews.org
Early detection of pests and pathogens is of paramount importance in reducing agricultural
losses. One approach to early detection is point-of-care (POC) diagnostics, which can …

Assessment of bacterial viability: a comprehensive review on recent advances and challenges

SS Kumar, AR Ghosh - Microbiology, 2019 - microbiologyresearch.org
Assessing bacterial contamination in environmental samples is critical in determining threats
to public health. The classical methods are time-consuming and only recognize species that …

Recent developments in the use of viability dyes and quantitative PCR in the food microbiology field

P Elizaquível, R Aznar, G Sánchez - Journal of applied …, 2014 - academic.oup.com
The increase in foodborne outbreaks highlights the need for rapid, sensitive and specific
methods for food safety monitoring, enabling specific detection and quantification of viable …