Partitioning in aqueous two-phase systems: fundamentals, applications and trends

AL Grilo, M Raquel Aires-Barros… - Separation & Purification …, 2016 - Taylor & Francis
Over the last years, aqueous two-phase systems (ATPS) regained an increasing interest due
to their potential in the downstream processing of biomolecules. After many years with only a …

Improved virus purification processes for vaccines and gene therapy

P Nestola, C Peixoto, RRJS Silva… - Biotechnology and …, 2015 - Wiley Online Library
The downstream processing of virus particles for vaccination or gene therapy is becoming a
critical bottleneck as upstream titers keep improving. Moreover, the growing pressure to …

Intrinsically disordered proteins: the dark horse of the dark proteome

P Kulkarni, VN Uversky - Proteomics, 2018 - Wiley Online Library
A good portion of the 'protein universe'embodies the 'dark proteome'. The latter comprises
proteins not amenable to experimental structure determination by existing means and …

Microfluidic generation of all‐aqueous double and triple emulsions

M Jeyhani, R Thevakumaran, N Abbasi, DK Hwang… - Small, 2020 - Wiley Online Library
Higher order emulsions are used in a variety of different applications in biomedicine,
biological studies, cosmetics, and the food industry. Conventional droplet generation …

Past, present, and future of affinity-based cell separation technologies

K Bacon, A Lavoie, BM Rao, M Daniele, S Menegatti - Acta biomaterialia, 2020 - Elsevier
Progress in cell purification technology is critical to increase the availability of viable cells for
therapeutic, diagnostic, and research applications. A variety of techniques are now available …

Aqueous biphasic extraction of metal ions: An alternative technology for metal regeneration

R Karmakar, K Sen - Journal of Molecular Liquids, 2019 - Elsevier
Aqueous biphasic systems (ABSs) had been perceived way back in 1896 when its mere
possibility was observed by Beijerinck. However, the immense capability of these systems …

Microcarrier-based stem cell bioprocessing: GMP-grade culture challenges and future trends for regenerative medicine

A Ornelas-González… - Critical Reviews in …, 2021 - Taylor & Francis
Recently, stem cell-based therapies have been proposed as an alternative for the treatment
of many diseases. Stem cells (SCs) are well known for their capacity to preserve themselves …

The solvent side of proteinaceous membrane-less organelles in light of aqueous two-phase systems

BY Zaslavsky, LA Ferreira, AL Darling… - International journal of …, 2018 - Elsevier
Water represents a common denominator for liquid-liquid phase transitions leading to the
formation of the polymer-based aqueous two-phase systems (ATPSs) and a set of the …

Complete process for the selective recovery of textile dyes using aqueous two-phase system

L Alencar, LMS Passos, MAR Martins… - Separation and …, 2020 - Elsevier
Environmental problems arising from the release of dyes through industrial aqueous
effluents are an emerging concern. To mitigate the problem, this work provides an …

Thermodynamic insights into phase behavior a new aqueous two-phase system at different temperatures: Experimental equilibria, data correlation and modeling

G Teymoornejad, M Jabbari - Journal of Molecular Liquids, 2023 - Elsevier
In the current research, a novel aqueous ternary-two-phase system (ATPS) containing a
deep eutectic solvent (DES formed by mixing choline chloride with polyethylene glycol 400) …