Comprehensive Understanding of Laboratory Evolution for Food Enzymes: From Design to Screening Innovations

J Wu, Z Wang, M Zeng, Z He, Q Chen… - Journal of Agricultural …, 2024 - ACS Publications
In the field of food processing, enzymes play a pivotal role in improving product quality and
flavor, and extending shelf life. However, the exposure of traditional food enzymes to high …

Unlocking the potential of enzyme engineering via rational computational design strategies

L Zhou, C Tao, X Shen, X Sun, J Wang, Q Yuan - Biotechnology Advances, 2024 - Elsevier
Enzymes play a pivotal role in various industries by enabling efficient, eco-friendly, and
sustainable chemical processes. However, the low turnover rates and poor substrate …

Engineering the Reaction Pathway of a Non-heme Iron Oxygenase Using Ancestral Sequence Reconstruction

D Yang, CH Chiang, T Wititsuwannakul… - Journal of the …, 2024 - ACS Publications
Non-heme iron (FeII), α-ketoglutarate (α-KG)-dependent oxygenases are a family of
enzymes that catalyze an array of transformations that cascade forward after the formation of …

Ancestral Sequence Reconstruction to Enable Biocatalytic Synthesis of Azaphilones

CH Chiang, Y Wang, A Hussain… - Journal of the …, 2024 - ACS Publications
Biocatalysis can be powerful in organic synthesis but is often limited by enzymes' substrate
scope and selectivity. Developing a biocatalytic step involves identifying an initial enzyme …

Ancestral Sequence Reconstruction for Designing Biocatalysts and Investigating their Functional Mechanisms

K Prakinee, S Phaisan, S Kongjaroon, P Chaiyen - JACS Au, 2024 - ACS Publications
Biocatalysis has emerged as a green approach for efficient and sustainable production in
various industries. In recent decades, numerous advancements in computational and …

Unspecific Peroxygenase Catalyzes Selective Remote‐Site Functionalizations

W Li, DL Davis, KJ Speina, CB Monroe… - …, 2024 - Wiley Online Library
We describe the discovery of an unspecific peroxygenase (UPO) variant that catalyzes the
remote‐site functionalization of halogenated and unsaturated hydrocarbons with high …

Biophysical interactions between self-sufficient cytochrome P450 from Tepidiphilus thermophilus and ilaprazole

J You, Y Hwang, Y Jeong, SJ Yeom, CH Yun… - Dalton …, 2025 - pubs.rsc.org
Proton pump inhibitors (PPIs), metabolized by cytochrome P450 (P450) enzymes, are widely
used to inhibit gastric acid secretion. This study investigated CYP116B46, a self-sufficient …

Ancestor-originated engineering of medium-chain alcohol dehydrogenase enhances its catalytic compatibility by balancing activity, stability, and selectivity trade-offs

J Wang, L Qin, J Gu, L Wu, M Zou, X Su, Y Xu, Y Nie - Molecular Catalysis, 2025 - Elsevier
The role of medium-chain alcohol dehydrogenases (MDR) in the preparation of chiral drug
intermediates is indispensable; however, limited activity and stability towards unnatural …

Ancestral Sequence Reconstruction Reveals Determinants of Regioselectivity in C(sp3)-H Oxyfunctionalization Reactions by CYP505Es

AC Ebrecht, JC Aschenbrenner, Y Gumulya… - ACS …, 2024 - ACS Publications
Regioselective C–H functionalization of fatty acids and alcohols is a challenging reaction,
especially in-chain/midchain hydroxylation. These hydroxy fatty acids or diols offer a …

Promiscuity guided evolution of a decarboxylative aldolase for synthesis of chiral tertiary alcohols

M Campbell, A Ohler, M McGill, A Buller - 2024 - chemrxiv.org
Enzymes play an increasingly important role in synthetic biology and organic synthesis.
Many potential applications benefit from promiscuous activity with a diverse array of …