Subcellular localization of RNAs has gained attention in recent years as a prevalent
phenomenon that influences numerous cellular processes. This is also evident for the large …

Fine-tuning cellular physiology in response to intracellular and environmental cues requires
precise temporal and spatial control of gene expression. High-resolution imaging …

The MS2–MCP system enables researchers to image multiple steps of the mRNA life cycle
with high temporal and spatial resolution. However, for short-lived mRNAs, the tight binding …

The spatial regulation of protein translation is an efficient way to create functional and
structural asymmetries in cells. Recent research has furthered our understanding of how …

Long noncoding RNAs (lncRNAs) comprise a diverse class of transcripts that structurally
resemble mRNAs but do not encode proteins. Recent genome-wide studies in humans and …

Fluorescence in situ hybridization (FISH) and digital imaging microscopy were modified to
allow detection of single RNA molecules. Oligodeoxynucleotide probes were synthesized …

ASH1 mRNA localizes to the bud tip in Saccharomyces cerevisiae to establish asymmetry of
HO expression, important for mating type switching. To visualize real time localization of the …

The localization of mRNAs to subcellular compartments provides a mechanism for
regulating gene expression with exquisite temporal and spatial control. Recent studies …

Mating type in Saccharomyces cerevisiae is determined by two nonhomologous alleles,
MAT a and MAT α. These sequences encode regulators of the two different haploid mating …

Although subcellular mRNA trafficking has been demonstrated as a mechanism to control
protein distribution, it is generally believed that most protein localization occurs subsequent …